Invitrogen™ CRYBA1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA528954

Catalog No. PIPA528954

Description

Recommended positive controls: 293T, A431, H1299, HeLa, Molt-4, Raji, mouse fetal eye. Predicted reactivity: Mouse (95%), Rat (95%), Xenopus laevis (84%), Dog (96%), Rabbit (96%), Chicken (84%), Rhesus Monkey (97%), Bovine (93%), Guinea pig (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta acidic group member, encodes two proteins (crystallin, beta A3 and crystallin, beta A1) from a single mRNA, the latter protein is 17 aa shorter than crystallin, beta A3 and is generated by use of an alternate translation initiation site. Deletion of exons 3 and 4 causes the autosomal dominant disease 'zonular cataract with sutural opacities'.

Specifications

• Antigen: CRYBA1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CRYBA1
• Gene Alias: BA3/A1; BA3A1C; beta crystallin A3 chain transcript CN; beta crystallin A3 chain transcript LAM; beta crystallin A3 chain transcript PS; beta crystallin A3 chain transcript TC; beta-A1-crystallin; beta-A3; beta-A3 crystallin; beta-A3/A1-crystallin; beta-crystallin A1; beta-crystallin A3; Beta-crystallin A3, isoform A1, Delta4 form; Beta-crystallin A3, isoform A1, Delta7 form; Beta-crystallin A3, isoform A1, Delta8 form; Cryb; CRYB1; Cryba1; crystallin beta A1; crystallin beta A3; crystallin beta A3/A1; crystallin, beta A1; crystallin, beta A3; CTRCT10; eye lens structural protein; truncated beta-crystallin A3
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12957, 1411
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 0.26 mg/mL
• Formulation: 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: P02525, P05813
• Gene Symbols: CRYBA1
• Immunogen: Full length human beta A3 Crystallin Recombinant protein.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov