Invitrogen™ CSF2RB Chimeric Recombinant Mouse Monoclonal Antibody (BION-1)

Mouse Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ MA548119

Catalog No. PIMA548119

Description

Specificity: Binds to domain 4 of the common beta-chain and recognises the same binding site used by IL-5, GM-CSF and IL-3, or binds to an epitope in close proximity to it. Residues M363, R364, E366 (B-C loop) and R418 (F-G loop) in the common beta-chain are important for BION-1 binding. Preparation: BALB/c mice were immunized intraperitonally with COS cells transfected DQP (Domain 4 of the common beta-chain) expression constructs. The immunizations were repeated 4 times at 2-week intervals. Four weeks after the final immunization, a mouse was boosted with COS transfectants intravenously. Three days later, splenocytes were harvested and fused with NS-1 myeloma cells. Hybridoma supernatants were screened on CHO DQP cells by flow cytometry, with untransfected CHO cells as a control. All antibodies were from single hybridoma clones as selected by a limiting dilution method. MoAbs were purified from ascites fluid or hybridoma supernatant by a protein A sepharose column.

The protein encoded by this gene is the common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. Defects in this gene have been reported to be associated with protein alveolar proteinosis (PAP).

Specifications

• Antigen: CSF2RB Chimeric
• Classification: Recombinant Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% ProClin 300
• Gene Accession No.: P32927
• Gene Symbols: Csf2rb
• Immunogen: BALB/c mice were immunized intraperitonally with COS cells transfected DQP (Domain 4 of the common beta-chain) expression constructs.
• Quantity: 200 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Functional Assay, Inhibition Assays, Immunohistochemistry, Immunoprecipitation, In vitro Assay
• Clone: BION-1
• Conjugate: Unconjugated
• Gene: Csf2rb
• Gene Alias: Ai2ca; AI848964; AIC2A; Aic2b; Bc; beta c; beta common cytokine receptor; BetaIl3; Bil3; CD antigen CD131; CD131; CD131 antigen; CDw131; colony stimulating factor 2 receptor beta common subunit; colony stimulating factor 2 receptor, beta 1, low-affinity (granulocyte-macrophage); colony stimulating factor 2 receptor, beta 2, low-affinity (granulocyte-macrophage); colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage); colony-stimulating factor 2 receptor subunit beta-2; colony-stimulating factor-2 receptor, beta, low-affinity; common beta chain; CSF2RB; Csf2rb1; Csf2rb2; Csfgmrb; cytokine receptor common subunit beta; GM-CSF/IL-3/IL-5 receptor common beta subunit; GM-CSF/IL-3/IL-5 receptor common beta-chain; IL-3 RB; IL-3 Receptor beta; IL-3 receptor class 2 subunit beta; Il3r; IL3R beta; IL-3R class 2 subunit beta; IL3RB; Il3rb1; Il3rb2; IL5RB; interleukin 3 common beta chain; interleukin 3 receptor/granulocyte-macrophage colony stimulating factor 3 receptor, beta (high affinity); interleukin-3 receptor class 2 subunit beta; Interleukin-3 receptor class II beta chain; SMDP5
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 1439
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid