Invitrogen™ CTCF Monoclonal Antibody (G.758.4)

Rabbit Monoclonal Antibody

Supplier:  Invitrogen™ MA511187

Catalog No. PIMA511187

Description

It is not recommended to aliquot this antibody. This antibody is not cross-reactive with BORIS.

Transcriptional factor CTCF is a ubiquitously expressed 11-zinc finger domain containing, DNA-binding nuclear phosphoprotein that is involved in multiple aspects of normal gene regulation including transcriptional repression and activation, gene silencing, chromatin insulation and regulation of imprinted sites. CTCF uses different combinations of ZF domains and interacts with the CCCTC motif on the DNA. It can bind to HAT- and HDAC-containing complexes thereby influencing transcriptional activation or repression respectively. It is a multifunctional protein involved in the transactivation of APPB gene, silencing of c-myc gene, and insulation of human beta-globin and DMI myotonic dystrophy locus and imprinting control of IGF2 and H19 regions. CTCF has also been shown to regulate the transcription of Pax6 and IRAK2 promoter thereby opening a therapeutic avenue. Mutations in this gene have been associated with invasive breast cancers, prostate cancers and Wilms' tumors.

Specifications

• Antigen: CTCF
• Classification: Monoclonal
• Concentration: 91 μg/mL
• Formulation: 0.01M HEPES with 100μg/mL BSA, 50% glycerol, 0.15M NaCl and <0.02% sodium azide; pH 7.5
• Gene Accession No.: P49711, Q61164, Q9R1D1
• Gene Symbols: CTCF
• Immunogen: Synthetic peptide corresponding to the carboxy terminus of the human CTCF protein.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Monkey
• Product Type: Antibody
• Isotype: IgG

• Applications: ChIP Assay, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: G.758.4
• Conjugate: Unconjugated
• Gene: CTCF
• Gene Alias: 11 zinc finger transcriptional repressor; 11-zinc finger protein; AW108038; CCCTC-binding factor; CCCTC-binding factor (zinc finger protein); Ctcf; ctcf protein; CTCFL paralog; im:7152666; MRD21; OTTHUMP00000197610; Transcriptional repressor CTCF; Unknown (protein for IMAGE:8125283)
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 10664, 13018, 83726
• Content And Storage: -20°C
• Form: Liquid