CTCF Polyclonal Antibody, Rockland™

Catalog No.	Quantity
50-199-2012	25 μL
50-199-2011	100 μg

Catalog No. 50-199-2012 Supplier Rockland Immunochemicals Supplier No. 600401C42S

Description

Rabbit Polyclonal Antibody

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This antibody reacts with endogenous CTCF protein. A BLAST analysis was used to suggest reactivity with CTCF from human, mouse, horse, bovine, panda, rabbit, Danio, and chicken based on a 100% homology with the immunizing sequence. Cross-reactivity with CTCF from other sources has not been determined.

Transcriptional factor CTCF is a ubiquitously expressed 11-zinc finger domain containing, DNA-binding nuclear phosphoprotein that is involved in multiple aspects of normal gene regulation including transcriptional repression and activation, gene silencing, chromatin insulation and regulation of imprinted sites. CTCF uses different combinations of ZF domains and interacts with the CCCTC motif on the DNA. It can bind to HAT- and HDAC-containing complexes thereby influencing transcriptional activation or repression respectively. It is a multifunctional protein involved in the transactivation of APPB gene, silencing of c-myc gene, and insulation of human beta-globin and DMI myotonic dystrophy locus and imprinting control of IGF2 and H19 regions. CTCF has also been shown to regulate the transcription of Pax6 and IRAK2 promoter thereby opening a therapeutic avenue. Mutations in this gene have been associated with invasive breast cancers, prostate cancers and Wilms' tumors.

Specifications

• Antigen: CTCF
• Applications: ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin), Western Blot
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene: CTCF
• Gene Accession No.: P49711, Q61164
• Gene Alias: 11 zinc finger transcriptional repressor; 11-zinc finger protein; AW108038; BOS_17095; CCCTC-binding factor; CCCTC-binding factor (zinc finger protein); CTCF; ctcf protein; CTCFL paralog; im:7152666; MRD21; OTTHUMP00000197610; Transcriptional repressor CTCF; Unknown (protein for IMAGE:8125283)
• Gene Symbols: CTCF
• Host Species: Rabbit
• Quantity: 25 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 10664, 13018
• Target Species: Human, Mouse
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid