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Gibco™ CTS™ StemFlex™ Medium

Catalog No. A5465001
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Quantity:
500 mL

CTS StemFlex Medium is a xeno-free medium designed for robust expansion of feeder-free pluripotent stem cells (PSCs) and supports stressful applications in translational and clinical applications.

CTS (Cell Therapy Systems) StemFlex Medium is a xeno-free medium designed for robust expansion of feeder-free pluripotent stem cells (PSCs). The optimized formulation supports stressful applications for translational and clinical research and offers a flexible feeding schedule. PSCs expanded in CTS StemFlex Medium maintain stable karyotype, pluripotency, and tri-lineage differentiation potential across multiple passages and cell lines.

CTS StemFlex Medium:

  • Delivers reliable performance for gene editing and single cell passaging.
  • Offers a flexible feeding schedule.
  • Supports the transition from other adherent culture media.
  • Facilitates regulatory filings with GMP manufacturing, traceability of raw materials, and regulatory documentation.

Robust recovery and clonal expansion

CTS StemFlex Medium promotes consistent growth across multiple passages and supports high viability post single-cell passaging. The medium delivers efficient recovery following electroporation and achieves editing efficiencies similar to research-use-only (RUO) StemFlex Medium. CTS StemFlex Medium supports clonal expansion while maintaining pluripotency and normal karyotype of edited clones.

Workflow flexibility

CTS StemFlex Medium supports a flexible feeding schedule, including weekend-free feeding, and is compatible with different matrices and passaging reagents.

Simple transition

Induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) grown in other adherent culture media can be transitioned to CTS StemFlex Medium within a few passages without the need for extensive optimization. Additionally, cells cultured in CTS StemFlex Medium can be adapted to CTS StemScale PSC Suspension Medium, helping achieve consistent fold expansion per passage in suspension culture.

GMP manufactured

Gibco CTS products are manufactured at a site that uses methods and controls that conform with cGMP for medical devices, 21 CFR Part 820, helping ensure consistent quality. Our FDA-registered manufacturing site is ISO 13485-certified. Specific intended-use statements, full documentation traceability, and convenient access to our Drug Master File (DMF) are available for CTS StemFlex Medium.

TRUSTED_SUSTAINABILITY

Specifications

Cell Line Embryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs)
Cell Type Embryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs)
Classification Xeno-free
Concentration CTS StemFlex Basal Medium (1X), CTS StemFlex Supplement (10X)
Form Liquid
Product Type Cell Culture Medium
Serum Level Serum-free
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives None
Without Additives No Phenol Red
For Use With (Application) Cell and Gene Therapy Research, Development, and Manufacturing
Manufacturing Quality ISO13485, MDSAP, FDA-registered, 21 CFR820
Quantity 500 mL
Shipping Condition CTS StemFlex Basal Medium - ambient, CTS StemFlex Supplement - dry ice
Format Bottle
Formulation Liquid
Content And Storage 450 mL CTS StemFlex Basal Medium: store at 2°C to 8°C, protect from light. 50 mL CTS StemFlex Supplement: store at -5°C to -20°C, protect from light.
Culture Type Adherent Cell Culture, Feeder-free Stem Cell Culture (Human, iPS - Induced Pluripotent Stem, Embryonic), Stem Cell (Human, iPS - Induced Pluripotent Stem, Embryonic)
Expression System None
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For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. CAUTION: Not intended for direct administration into humans or animals.

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