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Gibco™ CTS™ Viral Production Cells 2.0
Description
Gibco CTS Viral Production Cells 2.0 are a clonal cell line derived from the HEK293F cell line and are a core component of the CTS AAV-MAX Helper-Free AAV Production System. The cells have been adapted to the chemically defined CTS Viral Production Medium and are maintained in suspension culture in that medium. CTS Viral Production Cells 2.0 can be transfected using the CTS AAV-MAX Transfection Kit for adeno-associated virus (AAV) production.
Features of the CTS Viral Production Cells 2.0 include:
- Clonal, 293F-derived, high-producer cell line
- Absence of SV40 large T antigen
- Optimized for high-density, suspension culture (>12 million cells/mL) in chemically defined medium
- Enable production of high AAV titers, greater than 5 x1010 vg/mL (unpurified)
- Robust scalability and passage stability
CTS Viral Production Cells 2.0 are a fully documented cGMP cell line
- Fully documented, cGMP-banked with detailed cell line lineage history
- Extensive quality testing based on USP <1043> and European Pharmacopoeia (Ph. Eur.) 5.2.12 recommendations
- Flexible licensing options without the burden of downstream royalties
- Contact us (outlicensing@thermofisher.com) for more information and access
- Frozen cells are supplied in a vial containing 1.5 mL of cells at 1 x 107 viable cells/mL in CTS Viral Production Medium and 7.5% DMSO. Cells should be thawed directly into CTS Viral Production Medium for optimal performance.
The CTS AAV-MAX Helper-Free AAV Production System is a scalable, high-titer platform for suspension AAV production with research-grade (Cat. No. A51217) and GMP versions to enable seamless transition from research to commercial manufacturing. Other components of the CTS AAV-MAX system include CTS Viral Production Medium (A5144001), CTS AAV-MAX Transfection Kit (A5427701), CTS Viral-Plex Complexation Buffer (A5145401), and Viral Production Medium, AGT (A5147101).
Specifications
Specifications
| Content And Storage | 1 x 107 cells in a 2-mL vial. Store in vapor phase liquid nitrogen. |
| Cell Line | Viral Production Cells 2.0 |
| Cell Type | Kidney (Embryonic) |
| Culture Type | Suspension Cell Culture |
| Format | Cryopreserved vial |
| For Use With (Application) | Cell and Gene Therapy Research, Development, and Manufacturing |
| Media Recommendation | Gibco™ CTS™ Viral Production Medium |
| No. of Cells | See Certificate of Analysis |
| Product Type | Viral Production Cell Line |
| Quantity | 1.5 mL |
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Frequently Asked Questions (FAQs)
CTS Viral Production Cells 2.0 are specifically designed to work with CTS AAV MAX products to provide maximal adeno-associated virus (AAV) titers and optimal performance. CTS Viral Production Cells can be used but we don't recommend doing this as it will reduce system performance.
CTS Viral Production Cells 2.0 achieve maximum cell densities of >1.2 x 10E7 cells/mL in shake flask cultures.
We recommend using CTS Viral Production Cells 2.0 between passages 3 to 25 for adeno-associated virus (AAV) production.
We recommend allowing freshly thawed CTS Viral Production Cells 2.0 to recover in culture for at least three passages prior to transfection.
Yes, CTS Viral Production Cells 2.0 cells are a clonal cell line derived from single cell cloning of 293-F cells.
For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. CAUTION: Not intended for direct administration into humans or animals.
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