Invitrogen™ CUGBP1 Monoclonal Antibody (3B1)

Catalog No.	Quantity
AMPA6675	100 μL

Catalog No. AMPA6675 Supplier Invitrogen™ Supplier No. MA116675

Description

Mouse Monoclonal Antibody

Suggested positive control: antigen standard for CUGBP1 (transient overexpression lysate).

Myotonic dystrophy (MD) is an autosomal dominant neuromuscular disease that is associated with a (CTG)n repeat expansion in the 3'-untranslated region of the myotonin protein kinase (Mt-PK) gene. A (CUG) n oligonucleotides triplet repeat pre-mRNA/mRNA binding protein may play an important role in DM pathogenesis. HeLa cell protein, CUG-BP1, has been purified based upon its ability to bind specifically to (CUG) 8 oligonucleotides in vitro. CUG-BP1 is the major (CUG) 8 binding activity in normal cells. CUG-BP1 has been identified as isoforms of a novel heterogeneous nuclear ribonucleoprotein (hnRNP), hNab50. The CUG-BP/hNab50 protein is localized predominantly in the nucleus and is associated with polyadenylated RNAs in vivo. In vitro RNA-binding/photocrosslinking studies demonstrate that CUG-BP/hNab50 binds to RNAs containing the Mt-PK 3-UTR. The (CUG) n repeat region in Mt-PK mRNA is a binding site for CUG-BP/hNab50 in vivo, and triplet repeat expansion leads to sequestration of this hnRNP on mutant Mt-PK transcripts.

Specifications

• Antigen: CUGBP1
• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: 3B1
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: TBS with 0.02% sodium azide
• Gene: CELF1
• Gene Accession No.: P28659, Q4QQT3, Q92879
• Gene Alias: 1600010O03Rik; 50 kDa nuclear polyadenylated RNA-binding protein; AA407467; Brain protein F41; Brunol2; bruno-like 2; bruno-like protein 2; Celf1; CELF-1; CUG RNA-binding protein; CUG triplet repeat RNA-binding protein 1; CUG triplet repeat, RNA binding protein 1; CUG triplet repeat, RNA-binding protein 1; Cugbp; CUG-BP; CUG-BP- and ETR-3-like factor 1; CUGBP Ela; CUGBP Elav-like family member 1; CUGBP, Elav-like family member 1; CUGBP, Elav-like family member 1-like; CUGBP1; CUG-BP1; D2Wsu101e; Deadenylation factor CUG-BP; deadenylation factor EDEN-BP; EDEN-BP; EDEN-BP homolog; embryo deadenylation element binding protein; embryo deadenylation element-binding protein homolog; HNAB50; NAB50; NAPOR; nuclear polyadenylated RNA-binding protein, 50-kD; RNA-binding protein BRUNOL-2; RP23-147D3.7
• Gene Symbols: CELF1
• Host Species: Mouse
• Immunogen: Full-length human CUGBP1.
• Purification Method: Protein G
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 100354668, 100514512, 10658, 13046, 362160, 540475
• Target Species: Human, Mouse, Rat, Rabbit, Monkey, Bovine, Pig
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ