Invitrogen™ Cullin 2 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5120902

Catalog No. PIPA5120902

Description

Positive test controls include: PC-3, K-562, HL-60, 293T, OVCAR3. Immunogen sequence: LEYVERATWN DRFSDIYALC VAYPEPLGER LYTETKIFLE NHVRHLHKRV LESEEQVLVM YHRYWEEYSK GADYMDCLYR YLNTQFIKKN KLTEADLQYG YGGVDMNEPL MEIGELALDM WRKLMVEPLQ AILIRMLLRE IKNDRGGEDP NQKVIHGVIN SFVHVEQYKK KFPLKFYQEI FESPFLTETG EYYKQEASNL LQESNCSQYM EKVLGRLKDE EIRCRKYLHP SSYTKVIHEC QQRMVADHLQ FLHAECHNII RQEKKNDMAN M.

Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346). May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF). [UniProt].

Specifications

• Antigen: Cullin 2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Cul2
• Gene Alias: 1300003D18Rik; 4932411N15Rik; AI327301; CUL2; CUL-2; cullin 2; cullin-2; MGC131970; mKIAA4106; OTTHUMP00000019437; testis secretory sperm-binding protein Li 238E
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 8453
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot, Immunocytochemistry
• Concentration: 0.63 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: Q13617
• Gene Symbols: Cul2
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 40-310 of human Cullin 2 (NP_001185708.1).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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