Invitrogen™ Cullin 4B Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA529856

Catalog No. PIPA529856

Description

Recommended positive controls: Target recombinant protein. Predicted reactivity: Human (99%), Mouse (98%), Rat (99%), Zebrafish (93%), Xenopus laevis (90%), Cat (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. Required for ubiquitination of cyclin E, and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism. Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8.

Specifications

• Antigen: Cullin 4B
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CUL4B
• Gene Alias: 2700050M05Rik; AA409770; CUL4B; CUL-4B; cullin 4B; cullin-4B; EGK_20888; KIAA0695; mKIAA0695; MRXHF2; MRXS15; MRXSC; RGD1564494; SFM2
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 8450
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 0.33 mg/mL
• Formulation: 0.1M tris glycine with 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q13620
• Gene Symbols: CUL4B
• Immunogen: Recombinant protein fragment corresponding to a region within amino acids 592 and 887 of Human Cullin 4B.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG