Invitrogen™ CX3CL1 Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 500P9850UG

Catalog No. 500P9850UG

Description

AA Sequence of recombinant protein: QHHGVTKCNI TCSKMTSKIP VALLIHYQQN QASCGKRAII LETRQHRLFC ADPKEQWVKD AMQHLDRQAA ALTRNG. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Fractalkine (CX3CL1). Anti-Human Fractalkine (CX3CL1)-specific antibody was purified by affinity chromatography employing an immobilized Human Fractalkine (CX3CL1) matrix. Sandwich ELISA: To detect Human Fractalkine (CX3CL1) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Fractalkine (CX3CL1) (500-P98Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Fractalkine (CX3CL1). Western Blot: To detect hFractalkine by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hFractalkine is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Chemokines are a family of proteins associated with the trafficking of leukocytes in immune surveillance and inflammatory cell recruitment. They are classified based on the positions of key cysteine residues. CX3CL1 is a CX3C chemokine known to induce adhesion and migration of leukocytes mediated by a membrane-bound and soluble form respectively. Recent experiments have shown that CX3CL1 can suppress the production of nitrous oxide, interleukin-6, and TNF-a in activated microglia and neuronal cells, suggesting that it may act as an intrinsic inhibitor against neurotoxicity by activated microglia. Its receptor, CX3CR1, also functions as a co-receptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by CX3CL1.

Specifications

• Antigen: CX3CL1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Cx3cl1
• Gene Alias: A-152E5.2; AB030188; ABCD-3; Acc1; AI848747; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C; CX3C membrane-anchored chemokine; C-X3-C motif chemokine 1; C-X3-C motif chemokine ligand 1; Cx3cl1; CXC3; CXC3C; D8Bwg0439e; Fkn; Fractalkine; neurotactin; NTN; NTT; Processed fractalkine; SCYD1; small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin); small inducible cytokine subfamily D (Cys-X3-Cys), member-1; small inducible cytokine subfamily D, 1; small-inducible cytokine D1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 6376
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P78423
• Gene Symbols: Cx3cl1
• Immunogen: E.coli-derived Recombinant Human Fractalkine (CX3CL1).
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody