Invitrogen™ CXCL1 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 500P115BT500UG

Catalog No. 50-271-8700

Description

AA Sequence of recombinant protein: APIANELRCQ CLQTMAGIHL KNIQSLKVLP SGPHCTQTEV IATLKNGREA CLDPEAPLVQ KIVQKMLKGV PK. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine KC (CXCL1). Anti-Murine KC (CXCL1)-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Murine KC (CXCL1) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine KC (CXCL1) (500-P115) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine KC (CXCL1). Western Blot: To detect mKC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mKC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P115BT-1MG will be provided as 2 x 500 μg

CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.

Specifications

• Antigen: CXCL1
• Classification: Polyclonal
• Conjugate: Biotin
• Gene: Cxcl1
• Gene Alias: alpha-chemokine; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 1; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha); Cinc1; CINC-1; CXC; CXC motif chemokine; C-X-C motif chemokine 1; C-X-C motif chemokine ligand 1; CXCL; CXCL1; CXCL-1; Cytokine-induced neutrophil chemoattractant 1; fibroblast secretory protein; Fsp; Gro; GRO a; GRO α; Gro1; GRO1 oncogene; GRO1 oncogene (melanoma growth stimulating activity, alpha); GRO1 oncogene (melanoma growth-stimulating activity); GROa; GROalpha; GRO-alpha(1-73); GRO-alpha(4-73); GRO-alpha(5-73); GRO-alpha(6-73); Growth-regulated alpha protein; GROα; Hematopoietic synergistic factor; HSF; KC; KC(5-72); KC/GR)-alpha; KC/GRO-alpha; KC-T; melanoma growth stimulating activity, alpha; Melanoma growth stimulatory activity; melanoma growth stimulatory activity alpha; MGSA; MGSA alpha; MGSA-a; N51; NAP3; NAP-3; Neutrophil-activating protein 3; platelet-derived growth factor-inducible protein KC; SCYB1; secretory protein N51
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 14825
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P12850
• Gene Symbols: Cxcl1
• Immunogen: E.coli-derived Recombinant Murine KC (CXCL1).
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG