CXCR-4, Rabbit anti-Human, Polyclonal, Millipore Sigma™

Catalog No.	Quantity
ABN144925UL	25 μL

Catalog No. ABN144925UL Supplier MilliporeSigma Supplier No. ABN144925UL

Description

Rabbit Polyclonal Antibody

Anti-CXCR-4 Antibody, Cat. No. ABN1449, is a highly specific rabbit polyclonal antibody that targets human CXCR-4 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

C-X-C chemokine receptor type 4 (UniProt: P61073; also known as CXC-R4, CXCR-4, FB22, Fusin, HM89, LCR1, Leukocyte-derived seven transmembrane domain receptor, LESTR, Lipopolysaccharide-associated protein 3, LAP-3, LPS-associated protein 3, NPYRL, Stromal cell-derived factor 1 receptor, SDF-1 receptor, CD184) is encoded by the CXCR4 gene (Gene ID: 7852) in human. CXCR-4 is a receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium levels and enhancing MAPK1/MAPK3 activation. CXCR-4 is expressed in numerous tissues and two isoforms have been reported that are generated by alternative splicing. Isoform 1 is the predominant form in all tissues tested. CXCR-4 is rapidly phosphorylated on serine and threonine residues in the C-terminal upon agonist stimulation. Phosphorylation at Ser324 and Ser325 leads to recruitment of ITCH (E3 ubiquitin-protein ligase), ubiquitination and protein degradation. Sulfation on Tyr21 is shown to be essential for efficient binding of CXCL12/SDF-1alpha and it promotes its dimerization. Tyr7 and Tyr12 are sulfated in a sequential manner following complete sulfation of Tyr21. CXCR-4 acts as a co-receptor for human HIV-1 virus isolates (CD4 being the primary receptor) and as a primary receptor for HIV-2 isolates. CXCR-4 can also bind bacterial lipopolysaccharide (LPS) and mediate LPS-induced inflammatory response, including TNF-alpha secretion by monocytes. Mutations in CXCR4 gene cause WHIM syndrome that is characterized by neutropenia, hypogammaglobulinemia and extensive human papillomavirus (HPV) infection.

Specifications

• Antigen: CXCR-4
• Applications: Immunohistochemistry (Paraffin), Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected CXCR-4 in human bone marrow, human pancreas, human breast and human prostate tissues.
• Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Alias: C-X-C chemokine receptor type 4;CXC-R4;FB22;Fusin;HM89;LCR1;Leukocyte-derived seven transmembrane domain receptor;LESTR;Lipopolysaccharide-associated protein 3;LAP-3;LPS-associated protein 3;NPYRL;Stromal cell-derived factor 1 receptor;SDF-1 receptor;CD184
• Gene Symbols: CXCR4
• Host Species: Rabbit
• Immunogen: KLH-conjugated linear peptide corresponding to 12 amino acids from the N-terminal region of human CXCR-4.
• Molecular Weight of Antigen: ∽53 kDa observed; 39.75 kDa calculated. Calculated molecular weight is 40 kDa but we observed it larger at around ∽50 kDa.Uncharacterized bands may be observed in some lysate(s).
• Purification Method: Affinity Purified
• Quantity: 25 μL
• Regulatory Status: RUO
• Research Discipline: Neuroscience
• Primary or Secondary: Primary
• Test Specificity: This rabbit polyclonal antibody detects CXCR-4 in human cells. It targets an epitope within 12 amino acids from the N-terminal region. Immunogen sequence is conserved between isoforms 1 and 2. Hence, it may react with both isoforms.
• Target Species: Human
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.