Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Bioactive Small Molecules
  5. 7394300U

Tocris Bioscience™ Cy5B-trisulfo, NHS

Catalog No. 7394300U
Change view
Click to view available options
Quantity:
300 μg
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
73-943-00U 300 μg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
This item is not returnable. View return policy
Catalog No. 73-943-00U Supplier Tocris Bioscience™ Supplier No. 7394/300U
This item is not returnable. View return policy

Description

Restricted conformation cyanine dye with high quantum yield and long fluorescence lifetime for advanced microscopy applications

Key information: Cy5B-trisulfo, NHS is a far-red fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Particularly useful for smFRET experiments to reduce blinking and bleaching artefacts and maintain correct mean FRET efficiencies. Application: FRET, smFRET, FLIM, TIRF, and super resolution microscopy (SRM) techniques, including dSTORM. Provides enhanced spatial resolution in dSTORM experiments. Does not provide efficient blinking in conventional high-thiol dSTORM buffers; instead, sodium borohydride (NaBH4) addition is required following which SmLM experiments can be performed in PBS. Properties and Photophysical Data: Excitation and emission maxima (λ) are 669 nm and 685 nm, respectively; extinction coefficient = 241,000 M-1cm-1; quantum yield = 0.4; fluorescence lifetime = 1.85 ns. We suggest making up a 10 mM stock solution of this product in anhydrous DMSO. For more information on how Cy5B-trisulfo, NHS may be used, see our protocol: Conjugation Protocol for Amine Reactive Dyes .

Specifications

Quantity 300 μg
Target Fluorescent Dyes
Molecular Formula C47H52N4Na2O15S3
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.