Cyanine Sheep anti-Chemical, Biotin, Polyclonal, Rockland™

Sheep Polyclonal Antibody

Supplier:  Rockland Immunochemicals 600606209

Catalog No. 50-199-2577

Description

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Sheep Serum and cyanine conjugated IgG. Reactivity was observed against Cy2, Cy3 and Cy5. Reactivity against other cyanine analogs is unknown.

Cyanines are blue-green fluorescent dyes belonging to polymethine group, used in biotechnology for labeling and analysis. The spectrum for Cyanine ranges from IR (489/506) to UV (743/767). Cyanine dyes include Cy2, Cy3, Cy3B, Cy3.5, Cy5, Cy5.5 and Cy7. These dyes exhibit brighter and more stable fluorescence than traditional FITC, TRITC/Rhodamine dyes. Cy3 and Cy5 are most commonly used for 2 color detection in Flow Cytometry applications.

Specifications

• Antigen: Cyanine
• Classification: Polyclonal
• Conjugate: Biotin
• Gene Alias: Cy dye; Cyanine dye
• Quantity: 1 mg
• Primary or Secondary: Primary
• Product Type: Antibody
• Target Molecule: Chemical

• Applications: ELISA, Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
• Host Species: Sheep
• Regulatory Status: RUO
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Lyophilized