Invitrogen™ Cyclin A2 Monoclonal Antibody (E23.1)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA1154

Catalog No. PIMA1154

Description

MA1-154 detects Cyclin A in mammalian samples and has been successfully used in Immunohistochemistry (Frozen and Paraffin), Immunofluorescence, Immunoprecipitation and Western Blot procedures. Immunoprecipitation and Western Blot analysis with MA1-154 show the accumulation of a prominent band at ~50 kDa in camptothecin and hydroxyurea treated cells. In Immunofluorescence applications, MA1-154 shows nuclear translocation of Cyclin A in G2/M arrested cells as a result of camptothecin treatment.

Cyclins are regulatory subunits of the cyclin-dependent kinases (cdks) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdks. These cyclin / cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S phase and passage through G2-phase.

Specifications

• Antigen: Cyclin A2
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA, 30% glycerol and 0.05% sodium azide
• Gene Accession No.: P20248, P30274, P51943
• Gene Symbols: CCNA2
• Immunogen: Bovine Cyclin A fusion protein.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Monkey, Bovine
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: E23.1
• Conjugate: Unconjugated
• Gene: CCNA2
• Gene Alias: AA408589; Ccn1; Ccn-1; CCNA; CCNA2; ccna2.S; Cyca; CycA2; Cyclin A; cyclin A2; Cyclin A2 (Cyclin A); cyclin A2 S homeolog; Cyclin A-3; cyclin-A; Cyclin-A2; XELAEV_18009326mg
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 114494, 12428, 281667, 890
• Content And Storage: -20°C
• Form: Liquid