Cyclin B1 Antibody (CCNB1/1098) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2478930.2MG

Catalog No. NBP247893B

Description

Cyclin B1 Monoclonal antibody specifically detects Antigen in Human, Mouse samples. It is validated for Flow Cytometry, Immunofluorescence, Immunohistochemistry (Paraffin).

Specifications

• Antigen: Cyclin B1
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready
• Gene Alias: CCNB, cyclin B1, G2/mitotic-specific cyclin B1, G2/mitotic-specific cyclin-B1
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG1 κ

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
• Clone: CCNB1/1098
• Conjugate: Unconjugated
• Formulation: PBS with No Preservative
• Gene Symbols: CCNB1
• Immunogen: Recombinant human full-length CCNB1 protein
• Quantity: 0.2 mg
• Research Discipline: Cancer, Cell Biology, Cell Cycle and Replication, Mitotic Regulators, Tumor Suppressors
• Gene ID (Entrez): 891
• Target Species: Human, Mouse
• Form: Purified