Invitrogen™ Cyclin B1 Monoclonal Antibody (5G6)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA515714

Catalog No. PIMA515714

Description

MA5-15714 targets CCNB1 in indirect ELISA, FACS, IF, and WB applications and shows reactivity with Human and Rat samples. The MA5-15714 immunogen is purified recombinant fragment of human CCNB1 expressed in E. Coli. MA5-15714 detects CCNB1 which has a predicted molecular weight of approximately 60kDa.

Cyclins bind to and regulate the activity of the Cyclin dependent protein kinases (CDKs). Cyclin B1 or CCNB1 is a regulatory protein involved in mitosis. It is essential for the control of the cell cycle at the G2/M transition. Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. Cyclin B1 is not ubiquitinated during G2/M phase, resulting in its steady accumulation during G2 phase, followed by abrupt APC dependent destruction at the end of mitosis. Destruction of Cyclin B1 is required for cell cycle progression. Cyclin B1 is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.

Specifications

• Antigen: Cyclin B1
• Classification: Monoclonal
• Concentration: Conc. Not Determined
• Formulation: Ascites with 0.03% sodium azide
• Gene Accession No.: P14635, P30277
• Gene Symbols: Ccnb1
• Immunogen: Purified recombinant fragment of human CCNB1 expressed in E. Coli.
• Regulatory Status: RUO
• Gene ID (Entrez): 25203, 891
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: ELISA, Flow Cytometry, Western Blot, Immunocytochemistry
• Clone: 5G6
• Conjugate: Unconjugated
• Gene: Ccnb1
• Gene Alias: Ccn-2; CCNB; Ccnb1; Ccnb1-rs1; Ccnb1-rs13; Cycb; Cycb1; Cycb1-rs1; Cycb-4; Cycb-5; cyclin B; cyclin B1; cyclin B1, related sequence 1; cyclin B1, related sequence 13; G2/mitotic-specific cyclin B1; G2/mitotic-specific cyclin-B1; I79_013589; OTTHUMP00000221981; OTTHUMP00000223023
• Host Species: Mouse
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Rat
• Product Type: Antibody
• Isotype: IgG1