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Invitrogen™ Cyclin B1 Monoclonal Antibody (V152)
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Catalog No. PIMA1155
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100 μg
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PIMA1155 100 μg
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Catalog No. PIMA1155 Supplier Invitrogen™ Supplier No. MA1155
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Description

Mouse Monoclonal Antibody

MA1-155 detects Cyclin B1 in mammalian samples. MA1-155 has been successfully used in Immunohistochemistry (paraffin), Immunofluorescence, Immunoprecipitation, Flow Cytometry and Western Blot procedures. Immunoprecipitation and Western Blot analysis with MA1-155 show the accumulation of a prominent band at ~52 kDa in camptothecin and hydroxyurea treated cells. MA1-155 also detects additional unknown bands at ~35 and ~75 kDa. In Immunofluorescence applications, MA1-155 shows cyclin B1 co-localization with microtubules, whereas MA1-156 shows cyclin B2 staining consistent with the Golgi region. MA1-155 specifically detects Cyclin B1 in in whole cell lysates, but will also cross-react with purified recombinant human Cyclin B2 in Western Blot applications.

Cyclins bind to and regulate the activity of the Cyclin dependent protein kinases (CDKs). Cyclin B1 or CCNB1 is a regulatory protein involved in mitosis. It is essential for the control of the cell cycle at the G2/M transition. Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. Cyclin B1 is not ubiquitinated during G2/M phase, resulting in its steady accumulation during G2 phase, followed by abrupt APC dependent destruction at the end of mitosis. Destruction of Cyclin B1 is required for cell cycle progression. Cyclin B1 is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Cyclin B1
Applications Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
Classification Monoclonal
Clone V152
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation PBS with 1mg/mL BSA, 30% glycerol and 0.05% sodium azide
Gene Ccnb1
Gene Accession No. P14635, P24860, P30277, Q08301
Gene Alias Ccn-2; CCNB; Ccnb1; Ccnb1-rs1; Ccnb1-rs13; Cycb; Cycb1; Cycb1-rs1; Cycb-4; Cycb-5; cyclin B; cyclin B1; cyclin B1, related sequence 1; cyclin B1, related sequence 13; G2/mitotic-specific cyclin B1; G2/mitotic-specific cyclin-B1; I79_013589; OTTHUMP00000221981; OTTHUMP00000223023
Gene Symbols Ccnb1
Host Species Mouse
Immunogen Hamster Cyclin B1.
Purification Method Protein A
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 100770842, 25203, 268697, 891
Target Species Human, Mouse, Rat, Monkey, Hamster
Content And Storage -20°C
Product Type Antibody
Form Liquid
Isotype IgG1
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