Invitrogen™ Cyclin B1 Monoclonal Antibody (V152)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA1155

Catalog No. PIMA1155

Description

MA1-155 detects Cyclin B1 in mammalian samples. MA1-155 has been successfully used in Immunohistochemistry (paraffin), Immunofluorescence, Immunoprecipitation, Flow Cytometry and Western Blot procedures. Immunoprecipitation and Western Blot analysis with MA1-155 show the accumulation of a prominent band at ~52 kDa in camptothecin and hydroxyurea treated cells. MA1-155 also detects additional unknown bands at ~35 and ~75 kDa. In Immunofluorescence applications, MA1-155 shows cyclin B1 co-localization with microtubules, whereas MA1-156 shows cyclin B2 staining consistent with the Golgi region. MA1-155 specifically detects Cyclin B1 in in whole cell lysates, but will also cross-react with purified recombinant human Cyclin B2 in Western Blot applications.

Cyclins bind to and regulate the activity of the Cyclin dependent protein kinases (CDKs). Cyclin B1 or CCNB1 is a regulatory protein involved in mitosis. It is essential for the control of the cell cycle at the G2/M transition. Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. Cyclin B1 is not ubiquitinated during G2/M phase, resulting in its steady accumulation during G2 phase, followed by abrupt APC dependent destruction at the end of mitosis. Destruction of Cyclin B1 is required for cell cycle progression. Cyclin B1 is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.

Specifications

• Antigen: Cyclin B1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA, 30% glycerol and 0.05% sodium azide
• Gene Accession No.: P14635, P24860, P30277, Q08301
• Gene Symbols: Ccnb1
• Immunogen: Hamster Cyclin B1.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Monkey, Hamster
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: V152
• Conjugate: Unconjugated
• Gene: Ccnb1
• Gene Alias: Ccn-2; CCNB; Ccnb1; Ccnb1-rs1; Ccnb1-rs13; Cycb; Cycb1; Cycb1-rs1; Cycb-4; Cycb-5; cyclin B; cyclin B1; cyclin B1, related sequence 1; cyclin B1, related sequence 13; G2/mitotic-specific cyclin B1; G2/mitotic-specific cyclin-B1; I79_013589; OTTHUMP00000221981; OTTHUMP00000223023
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 100770842, 25203, 268697, 891
• Content And Storage: -20°C
• Form: Liquid