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Invitrogen™ Cyclin B2 Monoclonal Antibody (X29.2)
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Catalog No. PIMA1156
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100 μg
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PIMA1156 100 μg
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Catalog No. PIMA1156 Supplier Invitrogen™ Supplier No. MA1156
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Description

Mouse Monoclonal Antibody

MA1-156 detects Cyclin B2 in mammalian samples. MA1-156 has been successfully used in Immunofluorescence, Immunoprecipitation, IHC (P) and Western Blot procedures. Immunoprecipitation and Western Blot analysis with MA1-156 show the accumulation of a prominent band at ~51 kDa in camptothecin and hydroxyurea treated cells. MA1-156 also detects additional unknown band at ~80 kDa. In Immunofluorescence applications, MA1-156 shows cyclin B2 staining consistent with the Golgi region, whereas MA1-155 shows cyclin B1 co-localization with microtubules. MA1-156 reacts with Cyclin B2 from Xenopus laevis and mammalian sources. In Western blot applications, X29.2 also cross reacts with Cyclin B1.

Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Cyclin B2
Applications Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
Classification Monoclonal
Clone X29.2
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation PBS with 1 mg/mL BSA, 30% glycerol and 0.05% sodium azide
Gene Ccnb2
Gene Accession No. O95067, P13351, P30276
Gene Alias C-B2; CCNB2; ccnb2.S; Cycb2; cyclin B2; cyclin B2 S homeolog; cyclinb2; G2/mitotic-specific cyclin B2; G2/mitotic-specific cyclin-B2; HsT17299; XELAEV_18020679mg
Gene Symbols Ccnb2, ccnb2.S
Host Species Mouse
Immunogen Xenopus laevis cyclin B2.
Purification Method Protein A
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 12442, 397743, 9133
Target Species Human, Mouse, Monkey, Frog
Content And Storage -20°C
Product Type Antibody
Form Liquid
Isotype IgG1
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