Cyclin D1 Antibody (SPM587), Alexa Fluor™ 350, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP234816AF350

Catalog No. NB006903

Description

Cyclin D1 Monoclonal specifically detects Cyclin D1 in Human, Mouse, Rat, Monkey samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Cyclin D1
• Classification: Monoclonal
• Conjugate: Alexa Fluor 350
• Formulation: 50mM Sodium Borate with 0.05% Sodium Azide
• Gene Symbols: CCND1
• Immunogen: Human recombinant full length cyclin D1 protein (Uniprot: P24385)
• Quantity: 0.1 mL
• Research Discipline: Cancer, Cell Cycle and Replication, Core ESC Like Genes, mTOR Pathway, Stem Cell Markers, Wnt Signaling Pathway
• Test Specificity: Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
• Content And Storage: Store at 4°C in the dark.

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Clone: SPM587
• Dilution: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
• Gene Alias: B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, BCL1D11S287E, cyclin D1, cyclin D1 (PRAD1: parathyroid adenomatosis 1), G1/S-specific cyclin D1, G1/S-specific cyclin-D1, PRAD1 oncogene, PRAD1B-cell CLL/lymphoma 1, U21B31
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Primate
• Isotype: IgG2a κ