Invitrogen™ Cyclophilin A Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA1025

Catalog No. PA1025

Description

PA1-025 detects recombinant human cyclophilin A (CyPA), but does not detect endogenous levels of CyPA. PA1-025 also detects the CyPA protein from CHO cells. PA1-025 has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects a prominent ~18 kDa protein representing recombinant human CyPA. The PA1-025 immunogen is recombinant human CyPA expressed in E. coli.

Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. Within the cyclophilin family, there are several different proteins which show a high degree of homology including CyPA, CyPB and CyPC. CyPA, also termed CyP-18, is the most abundant and ubiquitous cyclophilin found in all vertebrate tissues and is present in T-cells.

Specifications

• Antigen: Cyclophilin A
• Classification: Polyclonal
• Formulation: PBS with 0.05% sodium azide
• Gene Accession No.: P14851, P17742, P62937
• Gene Symbols: Ppia
• Immunogen: Purified recombinant human CyPA.
• Quantity: 400 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Hamster
• Product Type: Antibody
• Isotype: IgG

• Applications: Western Blot, Immunocytochemistry
• Conjugate: Unconjugated
• Gene: Ppia
• Gene Alias: 2700098C05; Cphn; CYCA; cyclophilin; Cyclophilin A; Cyclosporin A-binding protein; CyP A; CyP-18; CYPA; CyP-A; CYPH; EC 5.2.1.8; epididymis secretory sperm binding protein Li 69p; HEL-S-69p; HGNC:9253; I79_011869; MGC117158; MGC12404; MGC23397; p1B15; p31; Peptidyl-prolyl cis-trans isomerase A; Peptidyl-prolyl cis-trans isomerase A, N-terminally processed; peptidylprolyl isomerase A; peptidylprolyl isomerase A (cyclophilin A); PPIA; PPIase; PPIase A; rotamase; rotamase A; SP18; T cell cyclophilin
• Host Species: Rabbit
• Purification Method: Ammonium sulfate precipitation
• Regulatory Status: RUO
• Gene ID (Entrez): 100769490, 268373, 5478
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid