Invitrogen™ Cyclophilin B Monoclonal Antibody (11C11)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA549248

Catalog No. PIMA549248

Description

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Positive Control - WB: human placenta tissue, U-87MG whole cell, HepG2 whole cell, Caco-2 whole cell, SW620 whole cell, PANC-1 whole cell, THP-1 whole cell, HEK293 whole cell, rat spleen tissue, rat lung tissue, mouse spleen tissue, mouse lung tissue. IHC: human oesophagus squama cancer tissue, human ovarian cancer tissue, human placenta tissue, human tonsil tissue, human lung cancer tissue. ICC/IF: U20S cell. Flow: U20S cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins which bind FK506 are termed FK506 Binding Proteins (FKBP's) and those which bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation. Thus, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins.

Specifications

• Antigen: Cyclophilin B
• Classification: Monoclonal
• Concentration: 500 μg/mL
• Formulation: PBS with 4mg trehalose and 0.05mg sodium azide
• Gene Accession No.: P23284, P24368, P24369
• Gene Symbols: PPIB
• Immunogen: E. coli-derived human Cyclophilin B recombinant protein (Position: K158-E216).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: 11C11
• Conjugate: Unconjugated
• Gene: PPIB
• Gene Alias: AA408962; AA553318; AI844835; Cphn2; Cphn-2; cyclophilin B; cyclophilin-like protein; Cyp B; CyP-20b; CYPB; CYP-S1; EC 5.2.1.8; epididymis secretory protein Li 39; HEL-S-39; OI9; Peptidyl-prolyl cis-trans isomerase B; peptidylprolyl isomerase B; peptidylprolyl isomerase B (cyclophilin B); PPIase; PPIase B; Ppib; Rotamase; Rotamase B; S-cyclophilin; S-cyclophylin; SCYLP
• Host Species: Mouse
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 19035, 5479, 64367
• Content And Storage: -20°C
• Form: Lyophilized