Cytiva CyDye™ DIFluors: Saturation Dye Labeling Kits

Description

Allow detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel

• Size- and charge-matched dyes enable co-migration of labeled samples within the gel
• Bright and highly sensitive dyes allow the use of the minimal labeling technique
• Minimal loss of signal during labeling, separation, and scanning
• No change in signal over wide pH range used during first-dimension (IEF) separation
• Discrete signal from each fluor with minimal cross-talk contributes to high accuracy

Contain 100nm each Cy3 and Cy5 saturation dyes sufficient for at least 12 labeling reactions, using as little as 5μg protein per reaction. 25-8009-84 contains an additional vial of Cy3 saturation dye (400nm) for labeling up to 500μg of protein in a preparative gel.

For applications where only small amounts of sample are available, for example, in Laser Capture Microdissection.

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Specifications

• Format: Solid
• For Use With (Application): The Scarce Sample Labeling Kit are designed to be used for applications only small amounts of sample are available, for example, in laser capture microdissection
• Quantity: 100 nM each of Cy3 and Cy5
• Product Type: Amersham CyDye DIGE Fluor Labeling Kit
• Kit Contents: 100nM CyDye DIGE Fluor Cy3 saturation dye for analytical labeling, 100nM CyDye DIGE Fluor Cy5 saturation dye for analytical labeling
• Content And Storage: -15°C to -30°C
• Color: Green, Red