Invitrogen™ CYP27A1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA575271

Catalog No. PIPA575271

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

CYP27A1 participates in the degradation of cholesterol to bile acids in both the classic and acidic pathways. [1] It is the initiating enzyme in the acidic pathway to bile acids, yielding oxysterols by introducing a hydroxyl group to the carbon at the 27 position in cholesterol. In the acidic pathway, it produces 27-hydroxycholesterol from cholesterol whereas in the classic or neutral pathway, it produces 3beta-hydroxy-5-cholestenoic acid. It is also involved in the metabolism of vitamin D3. While CYP27A1 is present in many different tissues, its function in these tissues is largely uncharacterized. In macrophages, 27-hydroxycholesterol generated by this enzyme may be helpful against the production of inflammatory factors associated with cardiovascular disease.

Specifications

• Antigen: CYP27A1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CYP27A1
• Gene Alias: 1300013A03Rik; 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase; 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase; 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 26-hydroxylase; 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase; cholestanetriol 26-monooxygenase; cholesterol 27 hydroxylase; CP27; CTX; CYP27; CYP27A1; cyt; cytochrome P450 27; cytochrome P450 family 27 subfamily A member 1; cytochrome P450, 27; cytochrome P450, 27a1; cytochrome P450, family 27, subfamily a, polypeptide 1; cytochrome P450, subfamily XXVIIA (steroid 27-hydroxylase, cerebrotendinous xanthomatosis), polypeptide 1; Cytochrome P-450C27/25; P450C27; Sterol 26-hydroxylase, mitochondrial; sterol 27-hydroxylase; Vitamin D(3) 25-hydroxylase
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 104086, 1593, 301517
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: P17178, Q02318, Q9DBG1
• Gene Symbols: CYP27A1
• Immunogen: Synthetic peptide corresponding to amino acids 100-150 of Human CYP27A1.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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