Cytochrome c Antibody (7H8.2C12), Biotin, Novus Biologicals™

Mouse Monoclonal Antibody has been used in 32 publications

Supplier:  Novus Biologicals NB10055775

Catalog No. NB10055775

Description

Cytochrome c Monoclonal specifically detects Cytochrome c in Human, Mouse, Rat, Drosophila, Rabbit samples. It is validated for Western Blot, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen, Flow (Intracellular).

Specifications

• Antigen: Cytochrome c
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Dilution: Western Blot 0.05-0.5 ug/ml, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen 1:10-1:500, Flow (Intracellular)
• Gene Alias: CYCHCS, cytochrome c, cytochrome c, somatic, THC4
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: An approx. 15 kDa band is observed. This antibody recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
• Content And Storage: Store at 4C. Do not freeze.
• Isotype: IgG2b κ

• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Flow Cytometry
• Clone: 7H8.2C12
• Conjugate: Biotin
• Gene Accession No.: P99999
• Gene Symbols: CYCS
• Immunogen: Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon CYT were used as the immunogen (Jemmerson et al. 1991). The antibody recognizes an epitope within amino acids 93-104 of pigeon cytochrome C based on competitive ELISA results (Jemmerson et al. 1991).
• Quantity: 0.1 mL
• Research Discipline: Apoptosis, Core ESC Like Genes, Lipid and Metabolism, Stem Cell Markers
• Gene ID (Entrez): 54205
• Target Species: Human, Mouse, Rat, Drosophila, Rabbit
• Form: Purified

For Research Use Only