Cytochrome c Antibody (7H8.2C12 + CYCS/1010), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP24444700	Novus Biologicals NBP2444470.02MG	0.02 mg
NBP24444701	Novus Biologicals NBP2444470.1MG	0.1 mg
NBP24444702	Novus Biologicals NBP2444470.2MG	0.2 mg

Description

Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Cytochrome c Monoclonal specifically detects Cytochrome c in Human, Rat samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Cytochrome c
• Concentration: 0.2mg/mL
• Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Apoptosis, Cellular Markers, Cholesterol Metabolism, Core ESC Like Genes, Lipid and Metabolism, Mitochondrial Markers, Stem Cell Markers
• Formulation: 1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: CYCHCS, cytochrome c, cytochrome c, somatic, THC4
• Gene Symbols: CYCS
• Isotype: IgG
• Purification Method: Protein A or G purified
• Test Specificity: Cytochrome C is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).

• Clone: 7H8.2C12 + CYCS/1010
• Dilution: Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Rat
• Gene Accession No.: P99999
• Gene ID (Entrez): 54205
• Immunogen: Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c (7H8.2C12); Recombinant full-length human CYCS protein (CYCS/1010)
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 15 kDa

For Research Use Only