Invitrogen™ Cytochrome C Monoclonal Antibody (2B5), FITC, eBioscience™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ BMS1037FI

Catalog No. 50-177-04

Description

Description: Cytochrome c was identified as a component required for the crucial steps in apoptosis, caspase-3 activation and DNA fragmentation. Cytochrome c was shown to redistribute from mitochondria to cytosol during apoptosis in intact cells. Mitochrondrial cytochrome c is a water-soluble protein of 15 kDa with a net positive charge, residing loosely attached in the mitochrondrial intermembrane space. Cytochrome c functions in the respiratory chain by interaction with redox partners. The release of cytochrome c into the cytosol leads to an activation of an apoptotic program via activation of a caspase dependent pathway. Cytochrome c achieves this goal by interaction with other cytosolic factors forming a complex (apoptosome) composed of cytochrome c, Apaf-1, dATP and Apaf-3/caspase 9. Bcl-2 on the other hand was shown to be able to prevent apoptosis by blocking the release of cytochrome c from mitochondria. Applications Tested: Flow Cytometry. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

Specifications

• Antigen: Cytochrome C
• Classification: Monoclonal
• Concentration: 0.1 mg/mL
• Formulation: TBS with 1% BSA and 0.02% sodium azide; pH 7.2-7.4
• Gene Accession No.: P99999
• Gene Symbols: Cycs
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 54205
• Content And Storage: 4°C, store in dark
• Form: Liquid

• Applications: Flow Cytometry
• Clone: 2B5
• Conjugate: FITC
• Gene: Cycs
• Gene Alias: CG17903; CG17903-PA; CG17903-PB; CRF; CYC; CYC2; Cycs; CYCSA; Cyct-c-p; Cyt c; cyt c-p; cyt.c; CytC; Cyt-c; CYTC2; Cyt-c2; CytC-2; cyt-c-p; cytC-p; Cyt-c-p-PA; Cyt-c-p-PB; Cytochrome c; cytochrome c DC4; Cytochrome c proximal; Cytochrome C, expressed in somatic tissues; cytochrome c, somatic; Cytochrome c-2; cytochrome c-like; cytochrome c-like protein; Cytochrome c-proximal; cytochrome-c-p; Cytochrome-c-proximal; DC4; dCyt.C-p; DMc01; Dmel\CG17903; Dmel_CG17903; hc1; HCS; l(2)k13905; lethal (2) k13905; LOC100341175; LOC100357840; MGC128356 protein; pDMc01; RH17228p; THC4
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a