Cytochrome c Antibody (SPM389), Alexa Fluor™ 350, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP247692AF350

Catalog No. NB007136

Description

Cytochrome c Monoclonal specifically detects Cytochrome c in Human, Mouse, Rat, Amphibian, Avian, Canine, Drosophila, Equine, Pigeon samples. It is validated for Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Cytochrome c
• Classification: Monoclonal
• Conjugate: Alexa Fluor 350
• Formulation: 50mM Sodium Borate with 0.05% Sodium Azide
• Gene Symbols: CYCS
• Immunogen: Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c (Uniprot: P99999)
• Quantity: 0.1 mL
• Research Discipline: Apoptosis, Cellular Markers, Cholesterol Metabolism, Core ESC Like Genes, Lipid and Metabolism, Mitochondrial Markers, Stem Cell Markers
• Test Specificity: It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This monoclonal antibody recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
• Content And Storage: Store at 4°C in the dark.

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunofluorescence
• Clone: SPM389
• Dilution: Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunofluorescence
• Gene Alias: CYCHCS, cytochrome c, cytochrome c, somatic, THC4
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Amphibian, Avian, Canine, Drosophila, Equine
• Isotype: IgG2b κ