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Cytokeratin 10/13 Antibody (SPM262), Novus Biologicals™
SDP

Catalog No. NBP24471000 Shop All R&D Systems Products
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NBP24471000 0.02 mg
NBP24471001 0.1 mg
NBP24471002 0.2 mg
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Catalog No. NBP24471000 Supplier Novus Biologicals Supplier No. NBP2447100.02MG
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Mouse Monoclonal Antibody

Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Cytokeratin 10/13 Monoclonal specifically detects Cytokeratin 10/13 in Human, Feline samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.

Specifications

Antigen Cytokeratin 10/13
Applications Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Classification Monoclonal
Clone SPM262
Concentration 0.2mg/mL
Conjugate Unconjugated
Dilution Western Blot 0.25 - 0.5 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Formulation 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias BCIE, BIE, CK10, CK-10, cytokeratin 10, Cytokeratin-10, EHK, K10keratosis palmaris et plantaris, keratin 10, keratin, type I cytoskeletal 10, keratin-10, KPP
Gene Symbols KRT10
Host Species Mouse
Immunogen Cytoskeletal preparation extracted from human ectocervical epithelium.
Purification Method Protein A or G purified
Quantity 0.02 mg
Regulatory Status RUO
Research Discipline Cytoskeleton Markers
Primary or Secondary Primary
Gene ID (Entrez) 3858
Test Specificity This antibody recognizes cytokeratin 10 (56.5kDa) and cytokeratin 13 (53kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this MAb serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.
Target Species Human, Feline
Content And Storage Store at 4C.
Form Purified
Isotype IgG2a κ
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