Invitrogen™ Cytokeratin 10/13 Monoclonal Antibody (EBS-IF-003)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA533317

Catalog No. PIMA533317

Description

EBS-IF-003 reacts with 53kDa (CK13) and 56.6kDa (CK10) cytokeratin proteins as indicated by immunoblotting. On formalin-fixed, paraffin-embedded tissue sections EBS-IF-003 recognizes only CK13. On cryostat sections EBS-IF-003 serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and noncornifying stratified epithelia and more differentiated cells of squamous carcinomas. In paraffin sections EBS-IF-003 does not stain CK10 positive, CK13 negative epithelia, as for example epidermis.

Cytokeratins(CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie. Skin) and non-keratinizing cells (ie. Mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different CKs in the molecular range of 40-70 k Da and isoelectric points of 5-805 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.

Specifications

• Antigen: Cytokeratin 10/13
• Classification: Monoclonal
• Concentration: 100 μg/mL
• Formulation: PBS with 0.02% sodium azide
• Gene Accession No.: P13645, P13646, Q6EIZ0
• Gene Symbols: KRT10, KRT13, LOC101087886
• Immunogen: Cytoskeleton preparation from HeLa cells.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Canine, Feline
• Product Type: Antibody
• Isotype: IgG2a κ

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
• Clone: EBS-IF-003
• Conjugate: Unconjugated
• Gene: KRT10
• Gene Alias: BCIE; BIE; CK10; CK-10; CK13; CK-13; cytokeratin 10; cytokeratin 13; cytokeratin-10; Cytokeratin-13; EHK; epithelial keratin 10; K10; K13; Ker10; keratin 10; keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris); keratin 10, type I; keratin 13; keratin 13, type I; keratin, type I cytoskeletal 10; Keratin, type I cytoskeletal 13; keratin-10; Keratin-13; KPP; KRT10; KRT13; WSN2
• Host Species: Mouse
• Purification Method: Purified
• Regulatory Status: RUO
• Gene ID (Entrez): 101085587, 101087886, 3858, 3860, 490983, 491006
• Content And Storage: 4°C, do not freeze
• Form: Liquid