Invitrogen™ Cytokeratin 10/13 Monoclonal Antibody (EBS-IF-003)

Catalog No.	Quantity
PIMA533317	100 μg

Catalog No. PIMA533317 Supplier Invitrogen™ Supplier No. MA533317

Description

Mouse Monoclonal Antibody

EBS-IF-003 reacts with 53kDa (CK13) and 56.6kDa (CK10) cytokeratin proteins as indicated by immunoblotting. On formalin-fixed, paraffin-embedded tissue sections EBS-IF-003 recognizes only CK13. On cryostat sections EBS-IF-003 serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and noncornifying stratified epithelia and more differentiated cells of squamous carcinomas. In paraffin sections EBS-IF-003 does not stain CK10 positive, CK13 negative epithelia, as for example epidermis.

Cytokeratins(CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie. Skin) and non-keratinizing cells (ie. Mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different CKs in the molecular range of 40-70 k Da and isoelectric points of 5-805 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.

Specifications

• Antigen: Cytokeratin 10/13
• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
• Classification: Monoclonal
• Clone: EBS-IF-003
• Concentration: 100 μg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.02% sodium azide
• Gene: KRT10
• Gene Accession No.: P13645, P13646, Q6EIZ0
• Gene Alias: BCIE; BIE; CK10; CK-10; CK13; CK-13; cytokeratin 10; cytokeratin 13; cytokeratin-10; Cytokeratin-13; EHK; epithelial keratin 10; K10; K13; Ker10; keratin 10; keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris); keratin 10, type I; keratin 13; keratin 13, type I; keratin, type I cytoskeletal 10; Keratin, type I cytoskeletal 13; keratin-10; Keratin-13; KPP; KRT10; KRT13; WSN2
• Gene Symbols: KRT10, KRT13, LOC101087886
• Host Species: Mouse
• Immunogen: Cytoskeleton preparation from HeLa cells.
• Purification Method: Purified
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 101085587, 101087886, 3858, 3860, 490983, 491006
• Target Species: Human, Canine, Feline
• Content And Storage: 4°C, do not freeze
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2a κ