Cytokeratin 10 Antibody (DE-K10) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NB254402100	Novus Biologicals NBP254402100UG	100 μg
NB254402200	Novus Biologicals NBP254402200UG	200 μg

Description

Cytokeratin 10 Monoclonal specifically detects Cytokeratin 10 in Human, Canine, Feline samples. It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Cytokeratin 10
• Concentration: 1 mg/mL
• Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), CyTOF
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cytoskeleton Markers
• Formulation: PBS with No Preservative
• Gene ID (Entrez): 3858
• Immunogen: Cytoskeletal preparation extracted from human ectocervical epithelium
• Primary or Secondary: Primary
• Content And Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 56.5 kDa

• Clone: DE-K10
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells, Immunocytochemistry/Immunofluorescence 0.5 - 1 ug/ml, Immunohistochemistry-Paraffin 0.1 - 0.2 ug/ml, CyTOF-ready
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Canine, Feline
• Gene Alias: BCIE, BIE, CK10, CK-10, cytokeratin 10, Cytokeratin-10, EHK, K10keratosis palmaris et plantaris, keratin 10, Keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris), keratin, type I cytoskeletal 10, keratin-10, KPP
• Gene Symbols: KRT10
• Isotype: IgG1 κ
• Purification Method: Protein A or G purified
• Test Specificity: This MAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.

For Research Use Only