Cytokeratin 14 Mouse, Clone: SPM263, Novus Biologicals™

Mouse Monoclonal Antibody has been used in 3 publications

Catalog Number	Mfr. No.	Quantity
NBP234355F	Novus Biologicals NBP2343550.02MG	0.02 mg
NBP234355A	Novus Biologicals NBP2343550.1MG	0.1 mg
NBP234355B	Novus Biologicals NBP2343550.2MG	0.2 mg

Description

Cytokeratin 14 Monoclonal specifically detects Cytokeratin 14 in Human, Mouse, Rat samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Flow (Intracellular).

Specifications

• Antigen: Cytokeratin 14
• Dilution: Western Blot 0.5-1.0ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug, Immunohistochemistry-Paraffin 1-2ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat
• Gene Accession No.: P02533
• Gene ID (Entrez): 3861
• Immunogen: A synthetic peptide of 15 amino acid from the C-terminus of human keratin 14
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 50 kDa

• Clone: SPM263
• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cytoskeleton Markers
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: CK14, CK-14, cytokeratin 14, cytokeratin-14, EBS3, EBS4, K14, keratin 14, keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner), keratin, type I cytoskeletal 14, Keratin-14, NFJ
• Gene Symbols: KRT14
• Isotype: IgG3
• Purification Method: Protein A purified
• Test Specificity: Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.