Cytokeratin 18 Antibody (KRT18/835), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP24494900	Novus Biologicals NBP2449490.02MG	0.02 mg
NBP24494901	Novus Biologicals NBP2449490.1MG	0.1 mg
NBP24494902	Novus Biologicals NBP2449490.2MG	0.2 mg

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Cytokeratin 18 Monoclonal specifically detects Cytokeratin 18 in Human, Bovine (Negative), Canine (Negative), Hamster (Negative), Porcine (Negative), Rat (Negative), Sheep (Negative) samples. It is validated for Western Blot, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Protein Array.

Specifications

• Antigen: Cytokeratin 18
• Concentration: 0.2mg/mL
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cancer, Cell Biology, Cellular Markers, Cytoskeleton Markers
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: Cell proliferation-inducing gene 46 protein, cell proliferation-inducing protein 46, CK-18, CYK18, cytokeratin 18, cytokeratin-18, K18, keratin 18, keratin, type I cytoskeletal 18, keratin-18
• Gene Symbols: KRT18
• Isotype: IgG1
• Purification Method: Protein A or G purified
• Test Specificity: This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

• Clone: KRT18/835
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Bovine (Negative), Canine (Negative), Hamster (Negative), Mouse (Negative), Porcine (Negative), Rat (Negative)
• Gene Accession No.: P05783
• Gene ID (Entrez): 3875
• Immunogen: Recombinant full-length human KRT18 protein
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 45 kDa

For Research Use Only