Invitrogen™ Cytokeratin 18 Monoclonal Antibody (4D11E4)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA515458

Catalog No. PIMA515458

Description

MA5-15458 targets Cytokeratin 18 in indirect ELISA, IHC and WB applications and shows reactivity with Human samples. The MA5-15458 immunogen is purified recombinant fragment of human Cytokeratin 18 (aa391-483) expressed in E. Coli.. MA5-15458 detects Cytokeratin 18 which has a predicted molecular weight of approximately 48kDa.

Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Further, cytokeratin 18 is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas, but not in squamous cell carcinomas. Hepatocellular carcinomas express only cytokeratins 8 and 18. Mutations in the cytokeratin 18 gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for the cytokeratin 18 gene. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Specifications

• Antigen: Cytokeratin 18
• Classification: Monoclonal
• Concentration: Conc. Not Determined
• Formulation: Ascites with 0.03% sodium azide
• Gene Accession No.: P05783, P05784
• Gene Symbols: KRT18
• Immunogen: Purified recombinant fragment of human Cytokeratin 18 (aa391-483) expressed in E. Coli.
• Regulatory Status: RUO
• Gene ID (Entrez): 16668, 3875
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot
• Clone: 4D11E4
• Conjugate: Unconjugated
• Gene: KRT18
• Gene Alias: cb83; Cell proliferation-inducing gene 46 protein; CK 18; CK18; CK-18; CYK18; cytokeratin 18; cytokeratin endo B; cytokeratin type -1; DAPK-1; Cytokeratin18; cytokeratin-18; dapk1; dorsal aorta proneprin kinesin-1; DreK18; Endo B; I79_023185; K 18; K18; keratin 18; keratin 18, type I; keratin 18a, tandem duplicate 1; keratin complex 1, acidic, gene 18; keratin D; Keratin, type I cytoskeletal 18; Keratin18; keratin-18; Kerd; KRT 18; Krt1-18; KRT18; krt18 {ECO:0000250; krt18a.1; LOC100008885; PIG46; sb:cb83; type I cytoskeletal 18; UniProtKB:P05783}; wu:fa13f02; wu:fb36b04
• Host Species: Mouse
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG2b

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov