Cytokeratin 18 Mouse, Clone: SPM510, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP234367F	Novus Biologicals NBP2343670.02MG	0.02 mg
NBP234367A	Novus Biologicals NBP2343670.1MG	0.1 mg
NBP234367B	Novus Biologicals NBP2343670.2MG	0.2 mg

Description

Cytokeratin 18 Monoclonal specifically detects Cytokeratin 18 in Human, Bovine (Negative), Canine (Negative), Hamster (Negative), Mouse (Negative), Porcine (Negative), Rat (Negative), Sheep (Negative) samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Cytokeratin 18
• Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Bovine (Negative), Canine (Negative), Hamster (Negative), Mouse (Negative), Porcine (Negative), Rat (Negative)
• Gene Accession No.: P05783
• Gene ID (Entrez): 3875
• Immunogen: Human breast cancer PMC 42 cells
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 45 kDa

• Clone: SPM510
• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cancer, Cell Biology, Cellular Markers, Cytoskeleton Markers
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: Cell proliferation-inducing gene 46 protein, cell proliferation-inducing protein 46, CK-18, CYK18, cytokeratin 18, cytokeratin-18, K18, keratin 18, keratin, type I cytoskeletal 18, keratin-18
• Gene Symbols: KRT18
• Isotype: IgG1 κ
• Purification Method: Protein A purified
• Test Specificity: This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.