Cytokeratin 18 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA5120073

Catalog No. PIPA5120073

Description

Positive test controls include: HeLa, HT-29, HepG2, K-562,Mouse liver, Mouse kidney. The target is usually found in the following locations: Cytoplasm, Nucleus, nucleolus, perinuclear region. Immunogen sequence: SHYFKIIEDL RAQIFANTVD NARIVLQIDN ARLAADDFRV KYETELAMRQ SVENDIHGLR KVIDDTNITR LQLETEIEAL KEELLFMKKN HEEEVKGLQA QIASSGLTVE VDAPKSQDLA KIMADIRAQY DELARKNREE LDKYWSQQIE ESTTVVTTQS AEVGAAETTL TELRRTVQSL EIDLDSMRNL KASLENSLRE VEARYALQME QLNGILLHLE SELAQTRAEG QRQAQEYEAL LNIKVKLEAE IATYRRLLED GEDFNLGDAL DSSNSMQTIQ KTTTRRIVDG KVVSETNDTK VLRH

Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Further, cytokeratin 18 is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas, but not in squamous cell carcinomas. Hepatocellular carcinomas express only cytokeratins 8 and 18. Mutations in the cytokeratin 18 gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for the cytokeratin 18 gene. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Specifications

• Antigen: Cytokeratin 18
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: KRT18
• Gene Alias: cb83; Cell proliferation-inducing gene 46 protein; CK 18; CK18; CK-18; CYK18; cytokeratin 18; cytokeratin endo B; cytokeratin type -1; DAPK-1; Cytokeratin18; cytokeratin-18; dapk1; dorsal aorta proneprin kinesin-1; DreK18; Endo B; I79_023185; K 18; K18; keratin 18; keratin 18, type I; keratin 18a, tandem duplicate 1; keratin complex 1, acidic, gene 18; keratin D; Keratin, type I cytoskeletal 18; Keratin18; keratin-18; Kerd; KRT 18; Krt1-18; KRT18; krt18 {ECO:0000250; krt18a.1; LOC100008885; PIG46; sb:cb83; type I cytoskeletal 18; UniProtKB:P05783}; wu:fa13f02; wu:fb36b04
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16668, 294853, 3875
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1.41 mg/mL
• Formulation: PBS with 50% glycerol and 0.01% thimerosal; pH 7.3
• Gene Accession No.: P05783, P05784, Q5BJY9
• Gene Symbols: KRT18
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 127-430 of human Cytokeratin 18 (KRT18) (NP_000215.1).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG