Invitrogen™ Cytokeratin 1/5/6/8 Monoclonal Antibody (PCK-26), FITC

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA125027

Catalog No. MA125027

Description

Western blot detects a band approximately 58 kDa (cytokeratin 5), 56 kD (cytokeratin 6) and 52 kD (cytokeratin 8). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Cytokeratins are a subclass of intermediate filament proteins that play a crucial role in the structural integrity and function of epithelial cells. They are grouped based on their molecular weight and isoelectric points into Type I (acidic) and Type II (basic to neutral) cytokeratins. Cytokeratin 1 (CK1) and Cytokeratin 5 (CK5) are primarily expressed in the basal layer of stratified epithelia, contributing to the mechanical stability and resilience of the tissue. CK5 is often co-expressed with CK14, marking basal cell populations, while CK6 is an inducible cytokeratin found in proliferative and regenerative epidermis, often co-expressed with CK16 and CK17. In the context of carcinomas, CK5/6 expression is utilized as a marker for squamous cell carcinomas and other squamous variants. Cytokeratin 8 (CK8) is a Type II cytokeratin that partners with Cytokeratin 18 (CK18) and is predominantly found in simple epithelial cells, playing a key role in the cytoskeletal organization and intracellular signaling pathways. The diagnostic utility of these cytokeratin's lies in their tissue-specific expression patterns, which are exploited in immunohistochemistry to differentiate between various types of epithelial carcinomas.

Specifications

• Antigen: Cytokeratin 1/5/6/8
• Classification: Monoclonal
• Concentration: 3 mg/mL
• Formulation: PBS with 1% BSA and 15mM sodium azide; pH 7.4
• Gene Accession No.: P02538, P05786, P05787, P11679, P13647, P50446, Q10758, Q4FZU2, Q5XQN5, Q6P6Q2, Q922U2
• Gene Symbols: KRT5, KRT6A, KRT8
• Immunogen: Cytokeratin from human epidermis.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Canine, Rabbit, Bovine, Sheep, Hamster, Pig, Feline, Chicken, Chicken, Guinea Pig, Reptile, Goat, Lizard
• Product Type: Antibody
• Isotype: IgG1

• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
• Clone: PCK-26
• Conjugate: FITC
• Gene: KRT5
• Gene Alias: 2310016L08Rik; 3300001P10Rik; 58 kDa cytokeratin; 60-kDa keratin; AA960620; AL022697; alpha keratin; AU019895; AW146334; Card2; CK5; CK-5; CK6A; CK-6A; CK6C; CK-6C; CK6D; CK-6D; CK-6E; CK8; CK-8; CYK8; CYKER; cytokeratin 5; cytokeratin 6A; cytokeratin 6C; cytokeratin 6D; cytokeratin 8; cytokeratin 8 (370 AA); cytokeratin endo A; cytokeratin type II, component III; cytokeratin-5; Cytokeratin-6A; cytokeratin-6C; Cytokeratin-6D; cytokeratin-6E; cytokeratin-8; Cytokeratin-A; DDD; DDD1; EBS2; EndoA; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; Hom s 5; K2C8; K5; K6A; K6a keratin; K6C; K6D; K8; kamp-keratin derived antimicrobial peptide; KDAMP; Ker2; KERA; keratin 5; keratin 5 (epidermolysis bullosa simplex Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; keratin 6 alpha; keratin 6A; keratin 6A, , type II; keratin 6A, type II; keratin 6B; keratin 8; keratin 8, type II; keratin complex 2, basic, gene 5; keratin complex 2, basic, gene 6a; keratin complex 2, basic, gene 6c; keratin complex 2, basic, gene 8; keratin complex 2, gene 6a; keratin IV; keratin K6; keratin K6h; keratin, epidermal type II, K6A; keratin, type II cytoskeletal 5; keratin, type II cytoskeletal 60 kDa, component III; keratin, type II cytoskeletal 6A; keratin, type II cytoskeletal 6C; keratin, type II cytoskeletal 8; keratin, type II cytoskeletal 8-like protein; keratin-5; keratin-6A; Keratin-6-alpha; keratin-6C; keratin-8; KO; Krt-2.8; Krt2-5; Krt2-6; Krt2-6a; Krt2-6c; Krt2-8; KRT5; KRT5A; Krt6; KRT6A; Krt6b; KRT6C; KRT6D; Krt8; LOC683313; LOW QUALITY PROTEIN: keratin, type II cytoskeletal 5; LOW QUALITY PROTEIN: keratin, type II cytoskeletal 8; mK6[a]; MK6a; mK6-alpha; PC3; Sak57; similar to keratin complex 2, basic, gene 6a; Tfip8; tuftelin interacting protein 8; type II alpha-keratin IIA; type II alpha-keratin IIC; type II cytokeratin A (no.8); type-II keratin Kb12; type-II keratin Kb5; Type-II keratin Kb6; type-II keratin Kb8
• Host Species: Mouse
• Purification Method: IgG fraction
• Regulatory Status: RUO
• Gene ID (Entrez): 100303605, 100348891, 100352658, 100687760, 100715346, 100715629, 100736243, 100759488, 101081312, 101100735, 101110495, 102174931, 102175207, 102182762, 110308, 120093743, 16687, 16691, 25626, 281268, 281269, 369017, 3852, 3853, 3856, 407779, 408041, 426896, 486513, 486523, 614456
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
• Form: Liquid

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov