Cytokeratin 6 Mouse, Clone: LHK6 (same as LHK6B), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP234232F	Novus Biologicals NBP2342320.02MG	0.02 mg
NBP234232A	Novus Biologicals NBP2342320.1MG	0.1 mg
NBP234232B	Novus Biologicals NBP2342320.2MG	0.2 mg

Description

Cytokeratin 6 Monoclonal specifically detects Cytokeratin 6 in Human, Mouse samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Cytokeratin 6
• Dilution: Western Blot 0.5-1.0ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse
• Gene Accession No.: P02538
• Gene ID (Entrez): 3853
• Immunogen: A synthetic peptide of 11 amino acids (GSSTIKYTTTS) from C-terminus of human keratin 6
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 56 kDa

• Clone: LHK6 (same as LHK6B)
• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cytoskeleton Markers, Stem Cell Markers
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: Allergen Hom s 5, CK6A, CK-6A, CK6C, CK6D, CK-6D, cytokeratin 6A, cytokeratin 6C, cytokeratin 6D, Cytokeratin-6A, Cytokeratin-6D, K6A, K6C, K6D, keratin 6A, keratin 6C, keratin 6D, keratin, epidermal type II, K6A, keratin, type II cytoskeletal 6A, Keratin-6A, KRT6C, KRT6D, Type-II keratin Kb6
• Gene Symbols: KRT6A
• Isotype: IgG2a κ
• Purification Method: Protein A purified
• Test Specificity: This MAb recognizes a protein of 56kDa, identified as cytokeratin 6 (CK6). In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and Cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human Cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies. Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express CK6 and CK16. CK6 is strongly expressed in about 75% of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation.