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Cytokeratin 8/18 Antibody (K8.8 + DC10), DyLight 680, Novus Biologicals™
SDP

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Catalog No. NBP234655FR Supplier Novus Biologicals Supplier No. NBP234655FR
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Description

Mouse Monoclonal Antibody

Cytokeratin 8/18 Monoclonal specifically detects Cytokeratin 8/18 in Human samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Flow (Intracellular).

Specifications

Antigen Cytokeratin 8/18
Applications Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Classification Monoclonal
Clone K8.8 + DC10
Conjugate DyLight 680
Dilution Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Intracellular)
Gene Alias CARD2, CK8, CK-8, CYK8cytokeratin 8, Cytokeratin-8, K2C8, K8cytokeratin-8, keratin 8, keratin, type II cytoskeletal 8, keratin-8, KO, Type-II keratin Kb8
Gene Symbols KRT8
Host Species Mouse
Immunogen Keratin preparation from a human carcinoma (K8.8); PMC-42 human breast carcinoma cells (DC10)
Purification Method Protein A or G purified
Quantity 0.1 mL
Regulatory Status RUO
Research Discipline Cancer, Cytoskeleton Markers
Primary or Secondary Primary
Gene ID (Entrez) 3856
Test Specificity Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This monoclonal antibody cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained. Immunohistochemical staining with this monoclonal antibody is indistinguishable from that obtained with monoclonal antibody 5D3.
Target Species Human
Content And Storage Store at 4C in the dark.
Form Purified
Isotype IgG1 κ
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