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  6. Cytokeratin 8/18 Antibody (K8.8 + DC10), Novus Biologicals™

Cytokeratin 8/18 Antibody (K8.8 + DC10), Novus Biologicals™
SDP

Catalog No. p-7105591 Shop All R&D Systems Products
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0.1 mg
0.2 mg
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Catalog No. Quantity
NBP234344F 0.02 mg
NBP234344A 0.1 mg
NBP234344B 0.2 mg
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Catalog No. NBP234344F Supplier Novus Biologicals Supplier No. NBP2343440.02MG
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Description

Mouse Monoclonal Antibody

Cytokeratin 8/18 Monoclonal specifically detects Cytokeratin 8/18 in Human samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Flow (Intracellular).

Specifications

Antigen Cytokeratin 8/18
Applications Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Classification Monoclonal
Clone K8.8 + DC10
Conjugate Unconjugated
Dilution Western Blot 0.5-1.0ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/mlimmunohistochemistry-Paraffin 0.5-1ug/ml, SDS-Page
Formulation PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Accession No. P05787
Gene Alias CARD2, CK8, CK-8, CYK8cytokeratin 8, Cytokeratin-8, K2C8, K8cytokeratin-8, keratin 8, keratin, type II cytoskeletal 8, keratin-8, KO, Type-II keratin Kb8
Gene Symbols KRT8
Host Species Mouse
Immunogen Keratin preparation from a human carcinoma (K8.8); PMC-42 human breast carcinoma cells (DC10)
Purification Method Protein A purified
Quantity 0.02 mg
Regulatory Status RUO
Research Discipline Cancer, Cytoskeleton Markers
Primary or Secondary Primary
Gene ID (Entrez) 3856
Test Specificity Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.
Target Species Human
Content And Storage Store at 4C.
Form Purified
Isotype IgG
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