Cytokeratin 8/18 Antibody (KRT8/803 + KRT18/835) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP247983A	Novus Biologicals NBP2479830.1MG	0.1 mg
NBP247983B	Novus Biologicals NBP2479830.2MG	0.2 mg

Description

Cytokeratin 8/18 Monoclonal specifically detects Cytokeratin 8/18 in Human samples. It is validated for Simple Western, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, CyTOF-ready.

Specifications

• Antigen: Cytokeratin 8/18
• Concentration: 1.0 mg/mL
• Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cancer, Cytoskeleton Markers
• Formulation: PBS with No Preservative
• Gene ID (Entrez): 3856
• Immunogen: Recombinant full-length human KRT8 protein (KRT8/803); Recombinant full-length human KRT18 protein (KRT18/835)
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

• Clone: KRT8/803 + KRT18/835
• Dilution: Western Blot : 0.5 - 1.0 ug/ml, Simple Western : 10 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Alias: CARD2, CK8, CK-8, CYK8cytokeratin 8, Cytokeratin-8, K2C8, K8cytokeratin-8, keratin 8, keratin, type II cytoskeletal 8, keratin-8, KO, Type-II keratin Kb8
• Gene Symbols: KRT8
• Isotype: IgG
• Purification Method: Protein A or G purified
• Test Specificity: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immuno-histochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.