Cytokeratin 8 Antibody (K8.8), DyLight 488, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP234654G

Catalog No. NBP234654G

Description

Cytokeratin 8 Monoclonal specifically detects Cytokeratin 8 in Human, Rat (Negative) samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Cytokeratin 8
• Classification: Monoclonal
• Conjugate: DyLight 488
• Gene Alias: CARD2, CK8, CK-8, CYK8cytokeratin 8, Cytokeratin-8, K2C8, K8cytokeratin-8, keratin 8, keratin, type II cytoskeletal 8, keratin-8, KO, Type-II keratin Kb8
• Host Species: Mouse
• Molecular Weight of Antigen: 52.5 kDa
• Quantity: 0.1 mL
• Research Discipline: Cancer, Cytoskeleton Markers
• Gene ID (Entrez): 3856
• Target Species: Human, Rat (Negative)
• Form: Purified

• Applications: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Clone: K8.8
• Dilution: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
• Gene Symbols: KRT8
• Immunogen: Keratin preparation from a human carcinoma (Uniprot: P05787)
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Epitope of this monoclonal antibody is located between aa343-357. Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular ('ring-like, perinuclear') from ductal ('peripheral-predominant') carcinoma of the breast.
• Content And Storage: Store at 4C in the dark.
• Isotype: IgG1 κ

For Research Use Only