Invitrogen™ Cytokeratin Pan Type I Monoclonal Antibody (AE1), eBioscience™, Invitrogen™

Catalog No.	Quantity
50-137-63	100 μg

Catalog No. 50-137-63 Supplier Invitrogen™ Supplier No. 14900182

Description

Mouse Monoclonal Antibody

Description: The monoclonal antibody AE1 recognizes many members of the acidic (Type I) family of cytokeratins. Cytokeratins are intermediate filament proteins comprising one component of the cytoskeleton. There are two large families of cytokeratins, acidic and basic, but all contain the same basic domains (i.e. an alpha-helical core with an N- and C-terminal domain). The proteins are expressed in epithelial cells, but are developmentally regulated. Many tumors also express these cytokeratins, and their expression can help identify the origin of a neoplasm. The AE1 monoclonal antibody recognizes 56.5, 54', 50, 50', 48, and 40 kDa proteins (also known as CK10, 14, 15, 16 and 19) in mouse, human, rat, primate (cynomolgus and rhesus), dog, cat, rabbit and chicken. Applications Reported: This AE1 antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, immunohistochemical staining, and immunocytochemistry. Applications Tested: This AE1 antibody has been tested by western blot on SDS-reduced lysates from MCF7 cells at less than or equal to 2 μg/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Specifications

• Antigen: Cytokeratin Pan Type I
• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: AE1
• Concentration: 0.5 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene: KRT16
• Gene Accession No.: O18740, O93256, P02533, P02535, P05783, P05784, P08727, P08779, P13645, P19001, P19012, P35527, Q5BJY9, Q61414, Q61781, Q63279, Q6EIZ0, Q6IFV1, Q6IFV3, Q6IFW6, Q6PVZ1, Q6RHW0, Q8CIS9, Q9Z2K1
• Gene Alias: 40-kDa keratin intermediate filament; 56 kDa cytokeratin; 67 kDa cytokeratin; AA960620; Acidic Cytokeratin; AI324768; AI528832; AI626930; AI663979; AL022697; alpha keratin; AU019895; BCIE; BIE; Card2; Cell proliferation-inducing gene 46 protein; CK1; CK-1; CK10; CK-10; CK14; CK-14; CK15; CK-15; CK16; CK-16; CK18; CK-18; CK19; CK-19; CK8; CK-8; CK-9; CYK18; CYK8; CYKER; cytokeratin 1; cytokeratin 10; cytokeratin 14; cytokeratin 15; cytokeratin 16; cytokeratin 18; cytokeratin 19; cytokeratin 8; cytokeratin 8 (370 AA); cytokeratin 9; cytokeratin endo A; Cytokeratin endo B; cytokeratin VIB; cytokeratin VII; Cytokeratin-1; Cytokeratin-10; cytokeratin-14; Cytokeratin-15; cytokeratin-16; Cytokeratin-18; cytokeratin-19; Cytokeratin-6B; cytokeratin-7; cytokeratin-8; Cytokeratin-9; cytokeratin-A; D130054E02Rik; EBS3; EBS4; EGK_03685; EHK; EHK1; Endo B; EndoA; EndoC; epidermal keratin 10; epidermal keratin VII; epidermolysis bullosa simplex, Dowling-Meara, Koebner; epidermolytic hyperkeratosis 1; epidermolytic hyperkeratosis; keratosis palmaris et plantaris; epithelial keratin 1; epithelial keratin 10; Epithelial keratin-1; EPPK; FNEPPK; focal non-epidermolytic palmoplantar keratoderma; GK-19; Hair alpha protein; HMWCK; intermediate filament protein; K1; K10; K14; K15; K16; K18; K19; K1C1; K1CO; K1CP; K1CS; K2C8; K8; K9; Ka10; Ka14; Ka15; Ka16; Ka19; Kb1; KER1; Ker10; KERA; keratin 1; keratin 1 (epidermolytic hyperkeratosis); keratin 1, type II; keratin 10; keratin 10 (epidermolytic hyperkeratosis); keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris); keratin 10, type I; keratin 13; keratin 14; keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner); keratin 14, type I; keratin 15; keratin 15, type I; keratin 16; keratin 16 (focal non-epidermolytic palmoplantar keratoderma); keratin 16, type I; keratin 18; keratin 18, type I; keratin 19; keratin 19, type I; keratin 24; keratin 8; keratin 8, type II; keratin 86; keratin 9; keratin 9 (epidermolytic palmoplantar keratoderma); keratin 9, type I; keratin complex 1, acidic, gene 10; keratin complex 1, acidic, gene 14; keratin complex 1, acidic, gene 15; keratin complex 1, acidic, gene 16; keratin complex 1, acidic, gene 18; keratin complex 1, acidic, gene 19; keratin complex 1, acidic, gene 9; keratin complex 2, basic, gene 1; keratin complex 2, basic, gene 8; keratin D; keratin intermediate filament 16a; keratin intermediate filament 16b; keratin, type I cytoskeletal 10; keratin, type I cytoskeletal 10-like; keratin, type I cytoskeletal 13; keratin, type I cytoskeletal 14; keratin, type I cytoskeletal 14; keratin, type I cytoskeletal 16; keratin, type I cytoskeletal 15; Keratin, type I cytoskeletal 16; keratin, type I cytoskeletal 18; Keratin, type I cytoskeletal 19; keratin, type I cytoskeletal 59 kDa; Keratin, type I cytoskeletal 9; keratin, type I, 40-kd; keratin, type II cytoskeletal 1; Keratin, type II cytoskeletal 8; keratin-1; Keratin-10; keratin-14; Keratin-15; keratin-15, basic; keratin-15, beta; keratin-16; Keratin-18; keratin-19; Keratin-8; keratin-9; Kerd; KO; KPP; KRT1; krt1 {ECO:0000250; Krt-1.10; Krt-1.14; Krt-1.19; KRT10; krt10 {ECO:0000250; Krt1-10; Krt1-14; Krt1-15; Krt1-16; Krt1-18; Krt1-19; KRT13; KRT14; Krt15; krt15 {ECO:0000250; Krt16; KRT16A; KRT18; krt18 {ECO:0000250; KRT19; Krt1-9; KRT1A; Krt-2.1; Krt-2.8; Krt2-1; KRT24; Krt2-8; Krt8; Krt86; krt9; KRTA; KRTB; LOW QUALITY PROTEIN: keratin, type I cytoskeletal 15; NEPPK; NFJ; pan keratin; pankeratin; PC1; PIG46; Spermatid perinuclear ring manchette protein K9; suprabasal cytokeratin 10; type I alpha-keratin 14; type I alpha-keratin 15; type I cytoskeletal 15; type I cytoskeletal 9; type I keratin KA10; Type I keratin Ka14; type I keratin KA15; type I keratin KA16; Type I keratin Ka19; type II cytokeratin A (no.8); type II keratin Kb1; type-II keratin Kb1; Type-II keratin Kb8; UniProtKB:P04264}; UniProtKB:P05783}; UniProtKB:P13645}; UniProtKB:P19012}; unnamed protein product
• Gene Symbols: KRT10, Krt14, KRT15, KRT16, KRT18, KRT19, KRT9
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 100426180, 100685323, 100685404, 100685481, 101749333, 106994041, 107656, 16661, 16664, 16665, 16666, 16668, 16669, 266717, 287700, 287701, 294853, 303530, 360626, 3857, 3858, 3861, 3866, 3868, 3875, 3880, 395861, 408039, 408040, 450225, 477601, 490978, 490980, 491006, 702153, 703932, 707236, 771977
• Target Species: Human, Mouse, Rat, Canine, Monkey, Chicken, Rhesus Macaque
• Content And Storage: 4°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1

For Research Use Only.