Tocris Bioscience™ DA ZP1

Description

Fluorogenic Zn(II) sensor for isolation of stem cell derived β cells

DA ZP1 (diacetylated Zinpyr1) is a fluorogenic Zn(II) sensor (Kd Zn2+ = 0.6 nM). It can be used to image and isolate pancreatic β-cells and β-like cells in vitro. Used to purify live stem cell-derived β-like cells. It can image transplanted islet grafts and endogenous mouse islets in vivo. DA ZP1 is non-fluorescent in the absence of Zn(II) ions but its binding with Zn(II) promotes hydrolytic cleavage of the acetyl groups, generating a strong fluorescent signal. Excitation and emission maxima (λ) are 490 nm and 522 nm, respectively.

Specifications

• Chemical Name or Material: 3 ',6 '-bis(Acetyloxy)-4 ',5 '-bis[[bis(2-pyridinylmethyl)amino]methyl]-2 ',7 '-dichlorospiro[isobenzofuran-1(3 H),9 '-[9 H]xanthen]-3-one
• CAS: 1816254-69-9
• Quantity: 2 mg
• Target: Fluorescent Cell Indicators and Sensors
• Molecular Formula: C₅₀H₄₀Cl₂N₆O₇
• Purity: 0.9