Invitrogen™ DAAM1 Monoclonal Antibody (5D3)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA547712

Catalog No. PIMA547712

Description

The antibody detects a 125 kDa* protein corresponding to the apparent molecular mass of DAAM1 on SDS-PAGE immunoblots of human A431, K562, and mouse C2C12 cells.

DAAM1 is a diaphanous-related formin first studied as a novel Disheveled binding protein and shown to be crucial for the planar cell polarity pathway in Xenopus. DAAM1, like other formins, directs nucleation and elongation of new actin filaments using its conserved formin-homology-2 (FH2) domain. It is a Rho-regulated formin implicated in neuronal development and regulation of gastrulation. It was identified as a binding protein of the Wnt receptor-associated protein Disheveled. It also binds the GTP-bound RhoA and increases its GTP-bound form population in cells. It exists in an autoinhibited state via intramolecular interactions between its amino-terminal GBD and carboxyl-terminal DAD domains. Structurally the protein includes a GTPase-binding domain (GBD), a diaphanous inhibitory domain (DID), and a coiled-coil region followed by the FH1, FH2 and C-terminal diaphanous auto-regulatory domain (DAD). In humans, the DAAM1 gene is mapped to chromosome 14q23.1.

Specifications

• Antigen: DAAM1
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene Accession No.: Q8BPM0, Q9Y4D1
• Gene Symbols: DAAM1
• Immunogen: Clone 5D3 was generated from a recombinant protein that included amino acid residues from the N-terminal region of human DAAM1. This sequence has less than 50% homology to the N-terminal region of DAAM2.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Western Blot, Immunocytochemistry
• Clone: 5D3
• Conjugate: Unconjugated
• Gene: DAAM1
• Gene Alias: Daam1; Disheveled-associated activator of morphogenesis 1; dishevelled associated activator of morphogenesis 1; I79_021650; KIAA0666
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 208846, 23002, 314212
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid