Invitrogen™ DAAM1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5109614

Catalog No. PIPA5109614

Description

Immunogen sequence: FKKKFAERVA EVKPKVEAIR SGSEEVFRSG ALKQLLEVVL AFGNYMNKGQ RGNAYGFKIS SLNKIADTKS SIDKNITLLH YLITIVENKY PSVLNLNEEL RDIPQAAKVN MTELDKEIST LRSGLKAVET ELEYQKSQPP QPGDKFVSVV SQFITVASFS FSDVEDLLAE AKDLFTKAVK HFGEEAGKIQ PDEFFGIFDQ FLQAVSEAKQ ENENMRKKKE EEERRARMEA QLKEQRERER KMRKAKENSE ESGEFDDLVS ALRSGEVFDK DLSKLKRNRK RITNQMTDSS RERPITKLNF.

DAAM1 is a diaphanous-related formin first studied as a novel Disheveled binding protein and shown to be crucial for the planar cell polarity pathway in Xenopus. DAAM1, like other formins, directs nucleation and elongation of new actin filaments using its conserved formin-homology-2 (FH2) domain. It is a Rho-regulated formin implicated in neuronal development and regulation of gastrulation. It was identified as a binding protein of the Wnt receptor-associated protein Disheveled. It also binds the GTP-bound RhoA and increases its GTP-bound form population in cells. It exists in an autoinhibited state via intramolecular interactions between its amino-terminal GBD and carboxyl-terminal DAD domains. Structurally the protein includes a GTPase-binding domain (GBD), a diaphanous inhibitory domain (DID), and a coiled-coil region followed by the FH1, FH2 and C-terminal diaphanous auto-regulatory domain (DAD). In humans, the DAAM1 gene is mapped to chromosome 14q23.1.

Specifications

• Antigen: DAAM1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: DAAM1
• Gene Alias: Daam1; Disheveled-associated activator of morphogenesis 1; dishevelled associated activator of morphogenesis 1; I79_021650; KIAA0666
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 208846, 23002, 314212
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 0.84 mg/mL
• Formulation: PBS with 50% glycerol and 0.01% thimerosal; pH 7.3
• Gene Accession No.: Q8BPM0, Q9Y4D1
• Gene Symbols: DAAM1
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 769-1068 of human DAAM1 (NP_001257449.1).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG