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Tocris Bioscience™ DAF FM diacetate
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Catalog No. 77561PACK
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1 Pack (10 x 50 μg)
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77-561-PACK 1 Pack (10 x 50 μg)
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Catalog No. 77-561-PACK Supplier Tocris Bioscience™ Supplier No. 7756/1 PACK
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Description

Cell permeable photostable nitric oxide (NO) fluorescent indicator

DAF-FM DA is a cell-permeable fluorescent probe for the detection of intracellular nitric oxide (NO). Once inside the cell, it is hydrolyzed by cytosolic esterases to a cell-impermeant DAF-FM. DAF-FM reacts with NO+ equivalents, such as nitric anhydride (N2O3), which are formed by autoxidation of NO. Under aerobic conditions, DAF-FM can rapidly and irreversibly trap NO to yield highly fluorescent triazolofluoresceins (DAF-Ts). DAF-FM DA is photostable and stable in pH 5.5 to 12. It can be used to quantitatively monitor agonist-evoked NO synthesis in cultured HUVECs. DAF-FM DA may also be used to quantify NO levels in induced pluripotent stem cell-derived derived cardiomyocytes (iPSC-CM).

It is recommended to prepare stock solutions in DMSO.

Specifications

Chemical Name or Material 4-Amino-5-methylamino-2',7'-difluorofluorescein diacetate
CAS 254109-22-3
Quantity 1 Pack (10 x 50 μg)
Target Fluorescent Cell Indicators and Sensors
Molecular Formula C25H18F2N2O7
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