Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Bioactive Small Molecules
  5. 77561PACK

Tocris Bioscience™ DAF FM diacetate
GREENER_CHOICE

Catalog No. 77561PACK
Click to view available options
Quantity:
1 Pack (10 x 50 μg)
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Cell permeable photostable nitric oxide (NO) fluorescent indicator

DAF-FM DA is a cell-permeable fluorescent probe for the detection of intracellular nitric oxide (NO). Once inside the cell, it is hydrolyzed by cytosolic esterases to a cell-impermeant DAF-FM. DAF-FM reacts with NO+ equivalents, such as nitric anhydride (N2O3), which are formed by autoxidation of NO. Under aerobic conditions, DAF-FM can rapidly and irreversibly trap NO to yield highly fluorescent triazolofluoresceins (DAF-Ts). DAF-FM DA is photostable and stable in pH 5.5 to 12. It can be used to quantitatively monitor agonist-evoked NO synthesis in cultured HUVECs. DAF-FM DA may also be used to quantify NO levels in induced pluripotent stem cell-derived derived cardiomyocytes (iPSC-CM).

It is recommended to prepare stock solutions in DMSO.

Specifications

Chemical Name or Material 4-Amino-5-methylamino-2',7'-difluorofluorescein diacetate
CAS 254109-22-3
Quantity 1 Pack (10 x 50 μg)
Target Fluorescent Cell Indicators and Sensors
Molecular Formula C25H18F2N2O7
Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Your feedback has been submitted: Thank you for helping us improve our website.