DC-SIGN/CD209 Mouse anti-Mouse, APC, Clone: MMD3, R&D Systems
Mouse Monoclonal Antibody
Manufacturer: R&D Systems FAB83451A025
DescriptionHuman DC-Sign (Dendritic Cell-Specific ICAM-3 Grabbing Nonintegrin), also known as CD209, is a member of the chromosome 19 C-type lectin family that includes DC-SIGN, DC-SIGN-related protein, CD23 and LSECtin (1). DC-SIGN was initially reported to be a 46kDa, 404 amino acid (aa) type II transmembrane protein that contained a 40 aa cytoplasmic N-terminus, a 21 aa transmembrane segment, and a 343 aa extracellular C-terminus (2). The extracellular region contains a distal, 115 aa Ca++-dependent carbohydrate-binding lectin domain and a membrane-proximal linker segment that is composed of seven 23 aa repeats (2, 3). The lectin domain is believed to preferably bind mannose, either within the context of ICAM-3 (on T cells) or ICAM-2 (on endothelial cells) (2, 4, 5). DC-SIGN expression appears to be limited to dendritic cells (DC) and macrophages (6), and DC interaction with the ICAMs both aids DC cell trafficking and immunological synapse formation (7). Since the original report on DC-SIGN, multiple splice forms have been discovered, generating both membrane-bound and soluble forms (3). There are eight type A isoforms, all of which begin with the same 15 aa of exon 1a. Four contain the transmembrane region of exon II, and four do not (i.e., are soluble). Among these eight type A isoforms, only three retain the entire 343 aa found in the full length form described in reference #2 (the full length form is referred to as type I mDC-SIGN1A) (3). Five additional isoforms utilize an alternate start site, and these are referred to as type B isoforms. These all show a 35 aa cytoplasmic domain. One also has a transmembrane segment; four do not. Two of the five contain full, unspliced extracellular regions (3). All of this suggests enormous complexity in DC-SIGN biology. DC-SIGN is not well conserved across species. Human and mouse show little overall aa sequence identity. In the lectin domain, however, human DC-SIGN shares 68% aa sequence identity with mouse DC-SIGN (8). Human and rhesus monkey DC-SIGN share 91% aa sequence identity over the entire extracellular region (8).
|Flow Cytometry 10 uL/10^6 cells|
|CD209, CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin, CD209 molecule, CDSIGNHIV gpl20-binding protein, CLEC4L, CLEC4LC-type lectin domain family 4 member L, DCSIGN, DC-SIGN1, DC-SIGN1C-type lectin domain family 4, member L, DC-SIGNMGC129965, Dendritic cell-specific ICAM-3-grabbing non-integrin 1|
|Dendritic Cell Marker|
|Supplied in a saline solution containing BSA and Sodium Azide. with Sodium Azide|
|Purified mouse DC-SIGN/CD209 extracellular domain Accession # FJ168685|
|Protein A or G purified from hybridoma culture supernatant|
|Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.|
|Detects mouse DC-SIGN/CD209 in direct ELISAs.|
We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.
Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.Ok