Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

DC-SIGN/CD209 Mouse anti-Mouse, APC, Clone: MMD3, R&D Systems™

Mouse Monoclonal Antibody

Manufacturer:  R&D Systems FAB83451A025

Catalog No. FAB83451A02


Add to cart
This item is not returnable. View return policy

Description

Description

Human DC-Sign (Dendritic Cell-Specific ICAM-3 Grabbing Nonintegrin), also known as CD209, is a member of the chromosome 19 C-type lectin family that includes DC-SIGN, DC-SIGN-related protein, CD23 and LSECtin (1). DC-SIGN was initially reported to be a 46kDa, 404 amino acid (aa) type II transmembrane protein that contained a 40 aa cytoplasmic N-terminus, a 21 aa transmembrane segment, and a 343 aa extracellular C-terminus (2). The extracellular region contains a distal, 115 aa Ca++-dependent carbohydrate-binding lectin domain and a membrane-proximal linker segment that is composed of seven 23 aa repeats (2, 3). The lectin domain is believed to preferably bind mannose, either within the context of ICAM-3 (on T cells) or ICAM-2 (on endothelial cells) (2, 4, 5). DC-SIGN expression appears to be limited to dendritic cells (DC) and macrophages (6), and DC interaction with the ICAMs both aids DC cell trafficking and immunological synapse formation (7). Since the original report on DC-SIGN, multiple splice forms have been discovered, generating both membrane-bound and soluble forms (3). There are eight type A isoforms, all of which begin with the same 15 aa of exon 1a. Four contain the transmembrane region of exon II, and four do not (i.e., are soluble). Among these eight type A isoforms, only three retain the entire 343 aa found in the full length form described in reference #2 (the full length form is referred to as type I mDC-SIGN1A) (3). Five additional isoforms utilize an alternate start site, and these are referred to as type B isoforms. These all show a 35 aa cytoplasmic domain. One also has a transmembrane segment; four do not. Two of the five contain full, unspliced extracellular regions (3). All of this suggests enormous complexity in DC-SIGN biology. DC-SIGN is not well conserved across species. Human and mouse show little overall aa sequence identity. In the lectin domain, however, human DC-SIGN shares 68% aa sequence identity with mouse DC-SIGN (8). Human and rhesus monkey DC-SIGN share 91% aa sequence identity over the entire extracellular region (8).
Specifications

Specifications

DC-SIGN/CD209
MMD3
Flow Cytometry 10 uL/10^6 cells
FJ168685
CD209, CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin, CD209 molecule, CDSIGNHIV gpl20-binding protein, CLEC4L, CLEC4LC-type lectin domain family 4 member L, DCSIGN, DC-SIGN1, DC-SIGN1C-type lectin domain family 4, member L, DC-SIGNMGC129965, Dendritic cell-specific ICAM-3-grabbing non-integrin 1
Mouse
IgG2c
25 Tests
Dendritic Cell Marker
Primary
30835
Mouse
Flow Cytometry
APC
Supplied in a saline solution containing BSA and Sodium Azide. with Sodium Azide
Purified
Cd209a
Purified mouse DC-SIGN/CD209 extracellular domain Accession # FJ168685
Protein A or G purified from hybridoma culture supernatant
RUO
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Monoclonal
Detects mouse DC-SIGN/CD209 in direct ELISAs.
SDS
Documents
Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit