Invitrogen™ DDX1 Monoclonal Antibody (2D4)

Catalog No.	Quantity
PIMA549263	100 μg

Catalog No. PIMA549263 Supplier Invitrogen™ Supplier No. MA549263

Description

Mouse Monoclonal Antibody

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Positive Control - WB: human K562 whole cell, human CACO-2 whole cell, human U20S whole cell, human HEK293 whole cell, human U87 whole cell, human HELA whole cell, human A549 whole cell, rat kidney tissue, mouse kidney tissue. IHC: human gastric cancer tissue, human pancreatic cancer tissue, rat brain tissue, human esophageal squamous carcinoma tissue. Flow: MCF-7 cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

DDX1 (ATP-dependent RNA helicase DDX1) acts as an ATP-dependent RNA helicase. It is able to unwind both RNA-RNA and RNA-DNA duplexes. DDX1 possesses 5' single-stranded RNA overhand nuclease activity and ATPase activity on various RNA, but not DNA polynucleotides. DDX1, together with RELA, acts as a coactivator to enhance NF-kappa-b-mediated transcriptional activation. It also acts as a positive transcriptional regulator of cyclin CCND2 expression. It binds to the cyclin CCND2 promoter region. DDX1 is a component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double stranded RNA and plays a role in activation of a cascade of antiviral responses including the induction of proinflammatory cytokines via the adapter molecule TICAM1.

Specifications

• Antigen: DDX1
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
• Classification: Monoclonal
• Clone: 2D4
• Concentration: 500 μg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 4mg trehalose and no preservative
• Gene: DDX1
• Gene Accession No.: Q641Y8, Q91VR5, Q92499
• Gene Alias: AA409185; ATP-dependent RNA helicase DDX1; DBP-RB; Ddx1; DEAD (Asp-Glu-Ala-Asp) box helicase 1; DEAD (Asp-Glu-Ala-Asp) box polypeptide 1; DEAD box polypeptide 1; DEAD box protein 1; DEAD box protein retinoblastoma; DEAD box-1; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1; DEAD/H-box helicase 1; UKVH5d
• Gene Symbols: DDX1
• Host Species: Mouse
• Immunogen: E.coli-derived human DDX1 recombinant protein (Position: K562-F740).
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 104721, 1653, 84474
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG2a