Invitrogen™ DDX23 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA534843

Catalog No. PIPA534843

Description

Recommended positive controls: HeLa, NIH-3T3, JC. Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (86%), Rhesus Monkey (100%), Bovine (100%). IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

PC4 (activated RNA polymerase II transcription cofactor 4) is a transcriptional coactivator, possessing the ability to suppress promoter-driven as well as nonspecific transcription via its DNA binding activity. The repressive activity of PC4 on promoter-driven transcription is alleviated by transcription factor TFIIH. TFIIH protects promoters from PC4-mediated repression by relieving the topological constraint imposed by PC4 through the ERCC3 helicase activity.

Specifications

• Antigen: DDX23
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Ddx23
• Gene Alias: 100 kDa U5 snRNP-specific protein; 3110082M05Rik; 4921506D17Rik; DDX23; DEAD (Asp-Glu-Ala-Asp) box polypeptide 23; DEAD box protein 23; DEAD-box helicase 23; probable ATP-dependent RNA helicase DDX23; prp28; PRP28 homolog; PRP28 homolog, yeast; PRP28p homolog; PRPF28; SNRNP100; U5 snRNP 100 kD protein; U5-100K; U5-100KD
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 74351, 9416
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q9BUQ8
• Gene Symbols: Ddx23
• Immunogen: Recombinant fragment corresponding to a region within amino acids 136 and 350 of DDX23 (Uniprot ID#Q9BUQ8).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG